Faculty Collaboration Database

Library Preparation for Multiplexed Reduced Representation Bisulfite Sequencing with a Universal Adapter. Methods Mol Biol 2019;2018:177-194

Date

06/23/2019

Pubmed ID

31228157

DOI

10.1007/978-1-4939-9581-3_8

Scopus ID

2-s2.0-85068180336

Abstract

Reduced representation bisulfite sequencing (RRBS) is an efficient approach for estimating cytosine methylation and hydroxymethylation at single nucleotide resolution with a focus on CpG sites located in CpG islands. Commonly used methods for multiplexing RRBS libraries involve a different indexed adapter, which is expensive to generate, for each library. Here, we describe a library preparation method that utilizes a universal adapter and labels samples with unique indexed PCR primers, significantly reducing the cost of multiplexed RRBS.

Author List

Liu Y, Kriegel AJ, Liang M

Authors

Alison J. Kriegel PhD Associate Professor in the Physiology department at Medical College of Wisconsin
Mingyu Liang PhD Center Director, Professor in the Physiology department at Medical College of Wisconsin
Yong Liu PhD Assistant Professor in the Physiology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

5-Methylcytosine
Animals
CpG Islands
Cytosine
DNA Methylation
DNA Primers
Gene Library
Rats
Sequence Analysis, DNA
Sulfites