Faculty Collaboration Database

Library Preparation for Multiplexed Reduced Representation Bisulfite Sequencing with a Universal Adapter. Methods Mol Biol 2019;2018:177-194

Date

06/23/2019

Pubmed ID

31228157

DOI

10.1007/978-1-4939-9581-3_8

Scopus ID

2-s2.0-85068180336 (requires institutional sign-in at Scopus site)   3 Citations

Abstract

Reduced representation bisulfite sequencing (RRBS) is an efficient approach for estimating cytosine methylation and hydroxymethylation at single nucleotide resolution with a focus on CpG sites located in CpG islands. Commonly used methods for multiplexing RRBS libraries involve a different indexed adapter, which is expensive to generate, for each library. Here, we describe a library preparation method that utilizes a universal adapter and labels samples with unique indexed PCR primers, significantly reducing the cost of multiplexed RRBS.

Author List

Liu Y, Kriegel AJ, Liang M

MESH terms used to index this publication - Major topics in bold

5-Methylcytosine
Animals
CpG Islands
Cytosine
DNA Methylation
DNA Primers
Gene Library
Rats
Sequence Analysis, DNA
Sulfites