Medical College of Wisconsin
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Immunologic response of the laryngeal mucosa to extraesophageal reflux. Ann Otol Rhinol Laryngol 2008 Dec;117(12):891-5



Pubmed ID




Scopus ID

2-s2.0-59649120967   10 Citations


OBJECTIVES: Extraesophageal reflux is common. The treatment costs are high, and there are associations with other diseases, including laryngeal cancer. Our studies of the mucosal immune response to this common inflammatory disease suggest an important role for the nonclassic antigen-presenting molecule CD1d in the response to inflammation. This study was performed to further explore the relationship between the CD1d-NKT cell-iGb3 axis and reflux.

METHODS: We carried out a prospective study of laryngeal biopsies from 12 patients with laryngopharyngeal reflux and 11 controls. Quantitative multiple-color immunofluorescence using antibodies for lymphocytes (CD3, CD161) and classic and nonclassic major histocompatibility complex (I, II, beta2m, CD1d) was performed, and univariate and multivariate analysis and co-localization measurements were applied.

RESULTS: Epithelial major histocompatibility complex class I and II expression was unchanged by reflux, but expression of CD1d increased (p < 0.05; luminal layers) and confidence intervals diminished in the reflux group. Co-localization of NKT cells with CD1d increased in patients (p < 0.01); iGb3 exhibited strong expression throughout all layers of the laryngeal epithelium.

CONCLUSIONS: These data indicate a role for the CD1d-NKT cell-iGb3 axis in response to extraesophageal reflux in humans. This represents a useful target for novel diagnostics and treatments for this common condition.

Author List

Birchall MA, Bailey M, Gutowska-Owsiak D, Johnston N, Inman CF, Stokes CR, Postma G, Pazmany L, Koufman JA, Phillips A, Rees LE


Nikki Johnston PhD Associate Professor in the Otolaryngology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Antigens, CD1d
Case-Control Studies
Fluorescent Antibody Technique
Gastroesophageal Reflux
Histocompatibility Antigens
Killer Cells, Natural
Laryngeal Mucosa
Middle Aged
NK Cell Lectin-Like Receptor Subfamily B
Prospective Studies
jenkins-FCD Prod-480 9a4deaf152b0b06dd18151814fff2e18f6c05280