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Nikki Johnston PhD

Nikki Johnston PhD profile photo picture

Associate Professor

Institution: Medical College of Wisconsin
Department: Otolaryngology
Program: Adult

Member of the Cancer Center
Member of the Genomic Sciences and Precision Medicine Center (GSPMC)

View Curriculum Vitae

Publications (70)

  • Pepsin in gastroesophageal and extraesophageal reflux: molecular pathophysiology and diagnostic utility. (Samuels TL, Johnston N) Curr Opin Otolaryngol Head Neck Surg 2020 Oct 16 PMID: 33060393 10/17/2020    
  • Analysis of Inflammatory Signaling in Human Middle Ear Cell Culture Models of Pediatric Otitis Media. (Espahbodi M, Samuels TL, McCormick C, Khampang P, Yan K, Marshall S, McCormick ME, Chun RH, Harvey SA, Friedland DR, Johnston N, Kerschner JE) Laryngoscope 2020 May 20 PMID: 32433794 SCOPUS ID: 2-s2.0-85085010563 05/21/2020    
  • Proximal reflux: biochemical mediators, markers, therapeutic targets, and clinical correlations. (Klimara MJ, Randall DR, Allen J, Figueredo E, Johnston N) Ann N Y Acad Sci 2020 May 13 PMID: 32401362 05/14/2020    
  • H+/K+ATPase Expression in the Larynx of Laryngopharyngeal Reflux and Laryngeal Cancer Patients. (McCormick CA, Samuels TL, Battle MA, Frolkis T, Blumin JH, Bock JM, Wells C, Yan K, Altman KW, Johnston N) Laryngoscope 2020 Apr 06 PMID: 32250454 SCOPUS ID: 2-s2.0-85083103241 04/07/2020    
  • RNA Sequencing Reveals Cancer-Associated Changes in Laryngeal Cells Exposed to Non-Acid Pepsin. (Samuels TL, Zimmermann MT, Zeighami A, Demos W, Southwood JE, Blumin JH, Bock JM, Johnston N) Laryngoscope 2020 Mar 23 PMID: 32202667 SCOPUS ID: 2-s2.0-85082674385 03/24/2020       1 Citation
  • Detection of Pepsin in Oral Secretions of Infants with and without Laryngomalacia. (Klimara MJ, Samuels TL, Johnston N, Chun RH, McCormick ME) Ann Otol Rhinol Laryngol 2020 Mar;129(3):224-229 PMID: 31631681 SCOPUS ID: 2-s2.0-85074441413 10/22/2019       1 Citation
  • Pepsin Triggers Neutrophil Migration Across Acid Damaged Lung Epithelium. (Hurley BP, Jugo RH, Snow RF, Samuels TL, Yonker LM, Mou H, Johnston N, Rosen R) Sci Rep 2019 09 24;9(1):13778 PMID: 31551494 PMCID: PMC6760148 SCOPUS ID: 2-s2.0-85072622956 09/26/2019       2 Citations
  • Correlation of salivary and nasal lavage pepsin with MII-pH testing. (Klimara MJ, Johnston N, Samuels TL, Visotcky AM, Poetker DM, Loehrl TA, Blumin JH, Bock JM) Laryngoscope 2020 04;130(4):961-966 PMID: 31329290 SCOPUS ID: 2-s2.0-85069855612 07/23/2019       1 Citation
  • Detection of pepsin and IL-8 in saliva of adult asthmatic patients. (Marshall S, McCann AJ, Samuels TL, Blair A, Bonne V, Johnston N, Koufman J) J Asthma Allergy 2019;12:155-161 PMID: 31213853 PMCID: PMC6549784 06/20/2019    
  • Esophageal pepsin and proton pump synthesis in barrett's esophagus and esophageal adenocarcinoma. (Samuels TL, Altman KW, Gould JC, Kindel T, Bosler M, MacKinnon A, Hagen CE, Johnston N) Laryngoscope 2019 12;129(12):2687-2695 PMID: 31046139 SCOPUS ID: 2-s2.0-85065403373 05/03/2019       5 Citations
  • The role of pepsin in epithelia-mesenchymal transition in idiopathic subglottic stenosis. (McCann AJ, Samuels TL, Blumin JH, Johnston N) Laryngoscope 2020 01;130(1):154-158 PMID: 30776094 SCOPUS ID: 2-s2.0-85061936635 02/19/2019       2 Citations
  • Pepsin: biomarker, mediator, and therapeutic target for reflux and aspiration. (Johnston N, Dettmar PW, Ondrey FG, Nanchal R, Lee SH, Bock JM) Ann N Y Acad Sci 2018 12;1434(1):282-289 PMID: 29774546 SCOPUS ID: 2-s2.0-85047661971 05/19/2018       7 Citations
  • Incidence and risk of aspiration in mechanically ventilated patients (Klimara MJ, Nanchal R, Johnston N) Reflux Aspiration and Lung Disease 1 January 2018:235-244 SCOPUS ID: 2-s2.0-85064371769 01/01/2018    
  • Association of Gel-Forming Mucins and Aquaporin Gene Expression With Hearing Loss, Effusion Viscosity, and Inflammation in Otitis Media With Effusion. (Samuels TL, Yan JC, Khampang P, Dettmar PW, MacKinnon A, Hong W, Johnston N, Papsin BC, Chun RH, McCormick ME, Kerschner JE) JAMA Otolaryngol Head Neck Surg 2017 08 01;143(8):810-817 PMID: 28594978 PMCID: PMC5710562 SCOPUS ID: 2-s2.0-85027729929 06/09/2017       9 Citations
  • Panel 8: Report on Recent Advances in Molecular and Cellular Biochemistry. (Preciado D, Granath A, Lin J, Val S, Kurabi A, Johnston N, Vijayasekaran S, Valdez T, Depireux D, Hermansson A) Otolaryngol Head Neck Surg 2017 04;156(4_suppl):S106-S113 PMID: 28372528 SCOPUS ID: 2-s2.0-85018984822 04/05/2017       2 Citations
  • Pepsin as a biomarker for laryngopharyngeal reflux in children with laryngomalacia. (Luebke K, Samuels TL, Chelius TH, Sulman CG, McCormick ME, Kerschner JE, Johnston N, Chun RH) Laryngoscope 2017 10;127(10):2413-2417 PMID: 28224634 SCOPUS ID: 2-s2.0-85013435856 02/23/2017       8 Citations
  • Airway reflux. (Johnston N, Ondrey F, Rosen R, Hurley BP, Gould J, Allen J, DelGaudio J, Altman KW) Ann N Y Acad Sci 2016 10;1381(1):5-13 PMID: 27310222 SCOPUS ID: 2-s2.0-84977120378 10/28/2016       22 Citations
  • Association of microRNA 146 with middle ear hyperplasia in pediatric otitis media. (Samuels TL, Yan J, Khampang P, MacKinnon A, Hong W, Johnston N, Kerschner JE) Int J Pediatr Otorhinolaryngol 2016 Sep;88:104-8 PMID: 27497395 PMCID: PMC4978186 SCOPUS ID: 2-s2.0-84979086846 08/09/2016       9 Citations
  • Gastroesophageal Reflux Burden, Even in Children That Aspirate, Does Not Increase Pediatric Hospitalization. (Duncan DR, Amirault J, Johnston N, Mitchell P, Larson K, Rosen RL) J Pediatr Gastroenterol Nutr 2016 08;63(2):210-7 PMID: 26794490 PMCID: PMC4917472 SCOPUS ID: 2-s2.0-84979732334 01/23/2016       11 Citations
  • Valproic acid suppresses the self-renewal and proliferation of head and neck cancer stem cells. (Lee SH, Nam HJ, Kang HJ, Samuels TL, Johnston N, Lim YC) Oncol Rep 2015 Oct;34(4):2065-71 PMID: 26239260 SCOPUS ID: 2-s2.0-84939160349 08/05/2015       6 Citations
  • The Impact of Pepsin on Human Nasal Epithelial Cells In Vitro: A Potential Mechanism for Extraesophageal Reflux Induced Chronic Rhinosinusitis. (Southwood JE, Hoekzema CR, Samuels TL, Wells C, Poetker DM, Johnston N, Loehrl TA) Ann Otol Rhinol Laryngol 2015 Dec;124(12):957-64 PMID: 26127000 SCOPUS ID: 2-s2.0-84953378153 07/02/2015       13 Citations
  • Local Synthesis of Pepsin in Barrett's Esophagus and the Role of Pepsin in Esophageal Adenocarcinoma. (Samuels T, Hoekzema C, Gould J, Goldblatt M, Frelich M, Bosler M, Lee SH, Johnston N) Ann Otol Rhinol Laryngol 2015 Nov;124(11):893-902 PMID: 26077392 PMCID: PMC4750403 SCOPUS ID: 2-s2.0-84952334416 06/17/2015       6 Citations
  • Association between tracheal pepsin, a reliable marker of gastric aspiration, and head of bed elevation among ventilated neonates. (Garland JS, Alex CP, Johnston N, Yan JC, Werlin SL) J Neonatal Perinatal Med 2014 Jan 01;7(3):185-92 PMID: 25318633 SCOPUS ID: 2-s2.0-84909981710 10/17/2014       8 Citations
  • Establishment of an immortalized laryngeal posterior commissure cell line as a tool for reflux research. (Lee SH, Samuels T, Bock JM, Blumin JH, Johnston N) Laryngoscope 2015 Feb;125(2):E73-7 PMID: 25272366 SCOPUS ID: 2-s2.0-84921691276 10/02/2014       7 Citations
  • Upper esophageal and pharyngeal cancers. (Bock JM, Howell AB, Johnston N, Kresty LA, Lew D) Ann N Y Acad Sci 2014 Sep;1325:49-56 PMID: 25266014 PMCID: PMC4617690 SCOPUS ID: 2-s2.0-84928795440 10/01/2014       1 Citation
  • Differential response of gel-forming mucins to pathogenic middle ear bacteria. (Kerschner JE, Hong W, Khampang P, Johnston N) Int J Pediatr Otorhinolaryngol 2014 Aug;78(8):1368-73 PMID: 24958163 PMCID: PMC4104932 SCOPUS ID: 2-s2.0-84904036054 06/25/2014       15 Citations
  • Chronic pepsin exposure promotes anchorage-independent growth and migration of a hypopharyngeal squamous cell line. (Kelly EA, Samuels TL, Johnston N) Otolaryngol Head Neck Surg 2014 Apr;150(4):618-24 PMID: 24376122 PMCID: PMC4423599 SCOPUS ID: 2-s2.0-84899057392 01/01/2014       18 Citations
  • Curcumin and anthocyanin inhibit pepsin-mediated cell damage and carcinogenic changes in airway epithelial cells. (Samuels TL, Pearson AC, Wells CW, Stoner GD, Johnston N) Ann Otol Rhinol Laryngol 2013 Oct;122(10):632-41 PMID: 24294686 SCOPUS ID: 2-s2.0-84887079765 12/04/2013       10 Citations
  • The utility of endoscopy and multichannel intraluminal impedance testing in children with cough and wheezing. (Rosen R, Amirault J, Johnston N, Haver K, Khatwa U, Rubinstein E, Nurko S) Pediatr Pulmonol 2014 Nov;49(11):1090-6 PMID: 24178927 11/02/2013    
  • Laryngopharyngeal reflux and GERD. (Johnston N, Dettmar PW, Strugala V, Allen JE, Chan WW) Ann N Y Acad Sci 2013 Oct;1300:71-79 PMID: 24117635 10/15/2013    
  • Prevalence of pediatric aspiration-associated extraesophageal reflux disease. (Kelly EA, Parakininkas DE, Werlin SL, Southern JF, Johnston N, Kerschner JE) JAMA Otolaryngol Head Neck Surg 2013 Oct;139(10):996-1001 PMID: 23989194 PMCID: PMC4764882 08/31/2013    
  • Extra-esophageal reflux, NOSE score, and sleep quality in an adult clinic population. (Laohasiriwong S, Johnston N, Woodson BT) Laryngoscope 2013 Dec;123(12):3233-8 PMID: 23754257 SCOPUS ID: 2-s2.0-84888428865 06/12/2013       8 Citations
  • The pathophysiology of gastroesophageal reflux (Johnston N) Gastroesophageal Reflux and the Lung 1 January 2013:23-41 SCOPUS ID: 2-s2.0-85028588374 01/01/2013    
  • Potential benefits of pH 8.8 alkaline drinking water as an adjunct in the treatment of reflux disease. (Koufman JA, Johnston N) Ann Otol Rhinol Laryngol 2012 Jul;121(7):431-4 PMID: 22844861 SCOPUS ID: 2-s2.0-84864130580 08/01/2012       22 Citations
  • Reproducibility of Salivary Pepsin: Clinic versus Home. (Laohasiriwong S, Johnston N, Woodson BT) Otolaryngol Head Neck Surg 2012 Aug;147(2 suppl):P195 PMID: 25718691 08/01/2012    
  • Extra-Esophageal Reflux (EER) and Sleep Disorders. (Laohasiriwong S, Woodson BT, Johnston N) Otolaryngol Head Neck Surg 2012 Aug;147(2 suppl):P120 PMID: 25718434 08/01/2012    
  • Pepsin promotes proliferation of laryngeal and pharyngeal epithelial cells. (Johnston N, Yan JC, Hoekzema CR, Samuels TL, Stoner GD, Blumin JH, Bock JM) Laryngoscope 2012 Jun;122(6):1317-25 PMID: 22570308 PMCID: PMC3816638 SCOPUS ID: 2-s2.0-84861808837 05/10/2012       55 Citations
  • The role of extraesophageal reflux in medically and surgically refractory rhinosinusitis. (Loehrl TA, Samuels TL, Poetker DM, Toohill RJ, Blumin JH, Johnston N) Laryngoscope 2012 Jul;122(7):1425-30 PMID: 22539181 SCOPUS ID: 2-s2.0-84862871424 04/28/2012       33 Citations
  • Reflux aspiration in children with neurodisability--a significant problem, but can we measure it? (Trinick R, Johnston N, Dalzell AM, McNamara PS) J Pediatr Surg 2012 Feb;47(2):291-8 PMID: 22325378 02/14/2012       22 Citations
  • Novel drug targets for reflux disease (Johnston N) Effects, Diagnosis and Management of Extra-Esophageal Reflux January 2012:335-345 SCOPUS ID: 2-s2.0-84895383079 01/01/2012    
  • Pepsin as a marker of extraesophageal reflux (Samuels TL, Johnston N) Effects, Diagnosis and Management of Extra-Esophageal Reflux January 2012:115-122 SCOPUS ID: 2-s2.0-84895377544 01/01/2012    
  • Effects, diagnosis and management of extra-esophageal reflux (Johnston N, Toohill RJ) Effects, Diagnosis and Management of Extra-Esophageal Reflux January 2012:1-530 SCOPUS ID: 2-s2.0-84895354820 01/01/2012    
  • Preface (Johnston N, Toohill RJ) Effects, Diagnosis and Management of Extra-Esophageal Reflux January 2012 SCOPUS ID: 2-s2.0-84895222649 01/01/2012    
  • The presence of pepsin in the lung and its relationship to pathologic gastro-esophageal reflux. (Rosen R, Johnston N, Hart K, Khatwa U, Nurko S) Neurogastroenterol Motil 2012 Feb;24(2):129-33, e84-5 PMID: 22141343 PMCID: PMC3307906 SCOPUS ID: 2-s2.0-84855967292 12/07/2011       35 Citations
  • Hoarseness and chronic cough: would you suspect reflux? (Sana S, Sana M, Johnston N, Mittal SK) J Fam Pract 2011 Aug;60(8):458-62 PMID: 21814640 SCOPUS ID: 2-s2.0-80053625397 08/05/2011       2 Citations
  • Higher rate of bronchoalveolar lavage culture positivity in children with nonacid reflux and respiratory disorders. (Rosen R, Johnston N, Hart K, Khatwa U, Katz E, Nurko S) J Pediatr 2011 Sep;159(3):504-6 PMID: 21777922 PMCID: PMC3157561 SCOPUS ID: 2-s2.0-80051792183 07/23/2011       12 Citations
  • Pepsin detection in patients with laryngopharyngeal reflux before and after fundoplication. (Wassenaar E, Johnston N, Merati A, Montenovo M, Petersen R, Tatum R, Pellegrini C, Oelschlager B) Surg Endosc 2011 Dec;25(12):3870-6 PMID: 21695583 06/23/2011       23 Citations
  • Evidence of extraesophageal reflux in idiopathic subglottic stenosis. (Blumin JH, Johnston N) Laryngoscope 2011 Jun;121(6):1266-73 PMID: 21557240 SCOPUS ID: 2-s2.0-79958258640 05/11/2011       49 Citations
  • Review article: Uptake of pepsin at pH 7 - In non-acid reflux - Causes inflammatory, and perhaps even neoplastic, changes in the laryngopharynx (Johnston N) Alimentary Pharmacology and Therapeutics April 2011;33(SUPPL. 1):13-20 SCOPUS ID: 2-s2.0-79952288013 04/01/2011    
  • Review article: Effects of pepsin and alginate in an animal model of squamous cell carcinoma (Allen J, Tinling SP, Johnston N, Belafsky P) Alimentary Pharmacology and Therapeutics April 2011;33(SUPPL. 1):21-28 SCOPUS ID: 2-s2.0-79952291445 04/01/2011    
  • Review article: reflux and its consequences--the laryngeal, pulmonary and oesophageal manifestations. Conference held in conjunction with the 9th International Symposium on Human Pepsin (ISHP) Kingston-upon-Hull, UK, 21-23 April 2010. (Pearson JP, Parikh S, Orlando RC, Johnston N, Allen J, Tinling SP, Johnston N, Belafsky P, Arevalo LF, Sharma N, Castell DO, Fox M, Harding SM, Morice AH, Watson MG, Shields MD, Bateman N, McCallion WA, van Wijk MP, Wenzl TG, Karkos PD, Belafsky PC) Aliment Pharmacol Ther 2011 Apr;33 Suppl 1:1-71 PMID: 21366630 SCOPUS ID: 2-s2.0-79958007520 03/05/2011       75 Citations
  • Analysis of pepsin in tracheoesophageal puncture sites. (Bock JM, Brawley MK, Johnston N, Samuels T, Massey BL, Campbell BH, Toohill RJ, Blumin JH) Ann Otol Rhinol Laryngol 2010 Dec;119(12):799-805 PMID: 21250551 01/22/2011    
  • Role of non-acidic reflux in laryngeal injury and disease (Johnston N) Laryngeal Diseases: Symptoms, Diagnosis and Treatments 2010:89-99 SCOPUS ID: 2-s2.0-84892974666 12/01/2010    
  • Rationale for targeting pepsin in the treatment of reflux disease. (Johnston N, Wells CW, Samuels TL, Blumin JH) Ann Otol Rhinol Laryngol 2010 Aug;119(8):547-58 PMID: 20860281 09/24/2010    
  • Pepsin as a marker of extraesophageal reflux. (Samuels TL, Johnston N) Ann Otol Rhinol Laryngol 2010 Mar;119(3):203-8 PMID: 20392035 04/16/2010    
  • Pepsin as a marker of extraesophageal reflux (Samuels TL, Johnston N) Annals of Otology, Rhinology and Laryngology March 2010;119(3):203-208 SCOPUS ID: 2-s2.0-77949427443 01/01/2010    
  • Pepsin as a causal agent of inflammation during nonacidic reflux. (Samuels TL, Johnston N) Otolaryngol Head Neck Surg 2009 Nov;141(5):559-63 PMID: 19861190 SCOPUS ID: 2-s2.0-70350501815 10/29/2009       87 Citations
  • Pepsin in nonacidic refluxate can damage hypopharyngeal epithelial cells. (Johnston N, Wells CW, Samuels TL, Blumin JH) Ann Otol Rhinol Laryngol 2009 Sep;118(9):677-85 PMID: 19810610 SCOPUS ID: 2-s2.0-70349438977 10/09/2009       79 Citations
  • Immunologic response of the laryngeal mucosa to extraesophageal reflux. (Birchall MA, Bailey M, Gutowska-Owsiak D, Johnston N, Inman CF, Stokes CR, Postma G, Pazmany L, Koufman JA, Phillips A, Rees LE) Ann Otol Rhinol Laryngol 2008 Dec;117(12):891-5 PMID: 19140534 SCOPUS ID: 2-s2.0-59649120967 01/15/2009       10 Citations
  • Mucin gene expression in human laryngeal epithelia: effect of laryngopharyngeal reflux. (Samuels TL, Handler E, Syring ML, Pajewski NM, Blumin JH, Kerschner JE, Johnston N) Ann Otol Rhinol Laryngol 2008 Sep;117(9):688-95 PMID: 18834073 SCOPUS ID: 2-s2.0-54249092323 10/07/2008       44 Citations
  • The mucosal immune response to laryngopharyngeal reflux. (Rees LE, Pazmany L, Gutowska-Owsiak D, Inman CF, Phillips A, Stokes CR, Johnston N, Koufman JA, Postma G, Bailey M, Birchall MA) Am J Respir Crit Care Med 2008 Jun 01;177(11):1187-93 PMID: 18323539 PMCID: PMC2643204 SCOPUS ID: 2-s2.0-44449125150 03/08/2008       26 Citations
  • Receptor-mediated uptake of pepsin by laryngeal epithelial cells. (Johnston N, Wells CW, Blumin JH, Toohill RJ, Merati AL) Ann Otol Rhinol Laryngol 2007 Dec;116(12):934-8 PMID: 18217514 SCOPUS ID: 2-s2.0-37349028008 01/26/2008       71 Citations
  • Role of extra-esophageal reflux in chronic otitis media with effusion. (Crapko M, Kerschner JE, Syring M, Johnston N) Laryngoscope 2007 Aug;117(8):1419-23 PMID: 17585281 SCOPUS ID: 2-s2.0-34548462940 06/23/2007       72 Citations
  • Activity/stability of human pepsin: implications for reflux attributed laryngeal disease. (Johnston N, Dettmar PW, Bishwokarma B, Lively MO, Koufman JA) Laryngoscope 2007 Jun;117(6):1036-9 PMID: 17417109 SCOPUS ID: 2-s2.0-34249873017 04/10/2007       148 Citations
  • Effect of pepsin on laryngeal stress protein (Sep70, Sep53, and Hsp70) response: role in laryngopharyngeal reflux disease. (Johnston N, Dettmar PW, Lively MO, Postma GN, Belafsky PC, Birchall M, Koufman JA) Ann Otol Rhinol Laryngol 2006 Jan;115(1):47-58 PMID: 16466100 SCOPUS ID: 2-s2.0-31144457906 02/10/2006       81 Citations
  • Laryngeal epithelial defenses against laryngopharyngeal reflux: investigations of E-cadherin, carbonic anhydrase isoenzyme III, and pepsin. (Gill GA, Johnston N, Buda A, Pignatelli M, Pearson J, Dettmar PW, Koufman J) Ann Otol Rhinol Laryngol 2005 Dec;114(12):913-21 PMID: 16425556 SCOPUS ID: 2-s2.0-29744443713 01/24/2006       77 Citations
  • Sensitive pepsin immunoassay for detection of laryngopharyngeal reflux. (Knight J, Lively MO, Johnston N, Dettmar PW, Koufman JA) Laryngoscope 2005 Aug;115(8):1473-8 PMID: 16094128 SCOPUS ID: 2-s2.0-23344449645 08/12/2005       90 Citations
  • Pepsin and carbonic anhydrase isoenzyme III as diagnostic markers for laryngopharyngeal reflux disease. (Johnston N, Knight J, Dettmar PW, Lively MO, Koufman J) Laryngoscope 2004 Dec;114(12):2129-34 PMID: 15564833 SCOPUS ID: 2-s2.0-10044275611 11/27/2004       146 Citations
  • Cell biology of laryngeal epithelial defenses in health and disease: further studies. (Johnston N, Bulmer D, Gill GA, Panetti M, Ross PE, Pearson JP, Pignatelli M, Axford SE, Dettmar PW, Koufman JA) Ann Otol Rhinol Laryngol 2003 Jun;112(6):481-91 PMID: 12834114 SCOPUS ID: 2-s2.0-0038516590 07/02/2003       138 Citations
  • Cell biology of laryngeal epithelial defenses in health and disease: preliminary studies. (Axford SE, Sharp N, Ross PE, Pearson JP, Dettmar PW, Panetti M, Koufman JA) Ann Otol Rhinol Laryngol 2001 Dec;110(12):1099-108 PMID: 11768697 01/05/2002       88 Citations
    • Last update: 10/25/2020
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