Cloning and characterization of mouse E2F8, a novel mammalian E2F family member capable of blocking cellular proliferation. J Biol Chem 2005 May 06;280(18):18211-20
Date
02/22/2005Pubmed ID
15722552DOI
10.1074/jbc.M501410200Scopus ID
2-s2.0-24044514663 (requires institutional sign-in at Scopus site) 156 CitationsAbstract
The E2F transcription factor family plays a crucial and well established role in cell cycle progression. Deregulation of E2F activities in vivo leads to developmental defects and cancer. Based on current evidence in the field, mammalian E2Fs can be functionally categorized into either transcriptional activators (E2F1, E2F2, and E2F3a) or repressors (E2F3b, E2F4, E2F5, E2F6, and E2F7). We have identified a novel E2F family member, E2F8, which is conserved in mice and humans and has its counterpart in Arabidopsis thaliana (E2Ls). Interestingly, E2F7 and E2F8 share unique structural features that distinguish them from other mammalian E2F repressor members, including the presence of two distinct DNA-binding domains and the absence of DP-dimerization, retinoblastoma-binding, and transcriptional activation domains. Similar to E2F7, overexpression of E2F8 significantly slows down the proliferation of primary mouse embryonic fibroblasts. These observations, together with the fact that E2F7 and E2F8 can homodimerize and are expressed in the same adult tissues, suggest that they may have overlapping and perhaps synergistic roles in the control of cellular proliferation.
Author List
Maiti B, Li J, de Bruin A, Gordon F, Timmers C, Opavsky R, Patil K, Tuttle J, Cleghorn W, Leone GAuthor
Gustavo Leone PhD Sr Associate Dean, Director, Professor in the Biochemistry department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Amino Acid SequenceAnimals
Cell Cycle Proteins
Cell Proliferation
Cells, Cultured
Cloning, Molecular
DNA-Binding Proteins
Dimerization
E2F Transcription Factors
E2F1 Transcription Factor
E2F2 Transcription Factor
E2F4 Transcription Factor
E2F5 Transcription Factor
E2F6 Transcription Factor
E2F7 Transcription Factor
Fibroblasts
Gene Library
Humans
Male
Mice
Molecular Sequence Data
Transcription Factors
