Diaphragm pico-liter pump for single-cell manipulation. Biomed Microdevices 2011 Aug;13(4):651-9
Date
04/16/2011Pubmed ID
21494795Pubmed Central ID
PMC6881176DOI
10.1007/s10544-011-9535-5Scopus ID
2-s2.0-80053911002 (requires institutional sign-in at Scopus site) 25 CitationsAbstract
A pico-liter pump is developed and integrated into a robotic manipulation system that automatically selects and transfers individual living cells of interest to analysis locations. The pump is a displacement type pump comprising one cylindrical chamber connected to a capillary micropipette. The top of the chamber is a thin diaphragm which, when deflected, causes the volume of the fluid-filled cylindrical chamber to change thereby causing fluid in the chamber to flow in and out of the micropipette. This enables aspirating and dispensing individual living cells. The diaphragm is deflected by a piezoelectric actuator that pushes against its center. The pump aspirates and dispenses volumes of fluid between 500 pL and 250 nL at flow rates up to 250 nL/s. The piezo-driven diaphragm arrangement provides exquisite control of the flow rate in and out of the capillary orifice. This feature, in turn, allows reduced perturbation of live cells by controlling and minimizing the applied shear stresses.
Author List
Anis Y, Houkal J, Holl M, Johnson R, Meldrum DAuthor
Roger H. Johnson PhD Associate Professor in the Biophysics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Biocompatible MaterialsCell Line
Equipment Design
Humans
Microfluidics
Models, Theoretical
Suction
