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Gene expression signatures of radiation response are specific, durable and accurate in mice and humans. PLoS One 2008 Apr 02;3(4):e1912

Date

04/03/2008

Pubmed ID

18382685

Pubmed Central ID

PMC2271127

DOI

10.1371/journal.pone.0001912

Scopus ID

2-s2.0-44849110695   90 Citations

Abstract

BACKGROUND: Previous work has demonstrated the potential for peripheral blood (PB) gene expression profiling for the detection of disease or environmental exposures.

METHODS AND FINDINGS: We have sought to determine the impact of several variables on the PB gene expression profile of an environmental exposure, ionizing radiation, and to determine the specificity of the PB signature of radiation versus other genotoxic stresses. Neither genotype differences nor the time of PB sampling caused any lessening of the accuracy of PB signatures to predict radiation exposure, but sex difference did influence the accuracy of the prediction of radiation exposure at the lowest level (50 cGy). A PB signature of sepsis was also generated and both the PB signature of radiation and the PB signature of sepsis were found to be 100% specific at distinguishing irradiated from septic animals. We also identified human PB signatures of radiation exposure and chemotherapy treatment which distinguished irradiated patients and chemotherapy-treated individuals within a heterogeneous population with accuracies of 90% and 81%, respectively.

CONCLUSIONS: We conclude that PB gene expression profiles can be identified in mice and humans that are accurate in predicting medical conditions, are specific to each condition and remain highly accurate over time.

Author List

Meadows SK, Dressman HK, Muramoto GG, Himburg H, Salter A, Wei Z, Ginsburg GS, Chao NJ, Nevins JR, Chute JP

Author

Heather A. Himburg PhD Associate Professor in the Radiation Oncology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Endotoxins
Environmental Exposure
Female
Gene Expression Profiling
Gene Expression Regulation
Humans
Lipopolysaccharides
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Radiation Tolerance
Radiation, Ionizing
Reproducibility of Results