Actomyosin is the main driver of interkinetic nuclear migration in the retina. Cell 2009 Sep 18;138(6):1195-208
Date
09/22/2009Pubmed ID
19766571Pubmed Central ID
PMC2791877DOI
10.1016/j.cell.2009.06.032Scopus ID
2-s2.0-70149124068 (requires institutional sign-in at Scopus site) 210 CitationsAbstract
Progenitor cell nuclei in the rapidly expanding epithelium of the embryonic vertebrate central nervous system undergo a process called interkinetic nuclear migration (IKNM). Movements of IKNM are generally believed to involve smooth migration of nuclei from apical to basal and back during the G1 and G2 phases of the cell cycle, respectively. Yet, this has not been formally demonstrated, nor have the molecular mechanisms that drive IKNM been identified. Using time-lapse confocal microscopy to observe nuclear movements in zebrafish retinal neuroepithelial cells, we show that, except for brief apical nuclear translocations preceding mitosis, IKNM is stochastic rather than smooth and directed. We also show that IKNM is driven largely by actomyosin-dependent forces as it still occurs when the microtubule cytoskeleton is compromised but is blocked when MyosinII activity is inhibited.
Author List
Norden C, Young S, Link BA, Harris WAAuthor
Brian A. Link PhD Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
ActomyosinAnimals
Cell Nucleus
Dynactin Complex
Embryo, Nonmammalian
Microtubule-Associated Proteins
Neuroepithelial Cells
Retina
Zebrafish
Zebrafish Proteins
