Medical College of Wisconsin
CTSICores SearchResearch InformaticsREDCap

Actomyosin is the main driver of interkinetic nuclear migration in the retina. Cell 2009 Sep 18;138(6):1195-208

Date

09/22/2009

Pubmed ID

19766571

Pubmed Central ID

PMC2791877

DOI

10.1016/j.cell.2009.06.032

Scopus ID

2-s2.0-70149124068   170 Citations

Abstract

Progenitor cell nuclei in the rapidly expanding epithelium of the embryonic vertebrate central nervous system undergo a process called interkinetic nuclear migration (IKNM). Movements of IKNM are generally believed to involve smooth migration of nuclei from apical to basal and back during the G1 and G2 phases of the cell cycle, respectively. Yet, this has not been formally demonstrated, nor have the molecular mechanisms that drive IKNM been identified. Using time-lapse confocal microscopy to observe nuclear movements in zebrafish retinal neuroepithelial cells, we show that, except for brief apical nuclear translocations preceding mitosis, IKNM is stochastic rather than smooth and directed. We also show that IKNM is driven largely by actomyosin-dependent forces as it still occurs when the microtubule cytoskeleton is compromised but is blocked when MyosinII activity is inhibited.

Author List

Norden C, Young S, Link BA, Harris WA

Author

Brian A. Link PhD Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Actomyosin
Animals
Cell Nucleus
Dynactin Complex
Embryo, Nonmammalian
Microtubule-Associated Proteins
Neuroepithelial Cells
Retina
Zebrafish
Zebrafish Proteins
jenkins-FCD Prod-486 e3098984f26de787f5ecab75090d0a28e7f4f7c0