Efficient Front-Rear Coupling in Neutrophil Chemotaxis by Dynamic Myosin II Localization. Dev Cell 2019 Apr 22;49(2):189-205.e6
Date
04/25/2019Pubmed ID
31014479Pubmed Central ID
PMC6708378DOI
10.1016/j.devcel.2019.03.025Scopus ID
2-s2.0-85064090810 (requires institutional sign-in at Scopus site) 50 CitationsAbstract
Efficient chemotaxis requires rapid coordination between different parts of the cell in response to changing directional cues. Here, we investigate the mechanism of front-rear coordination in chemotactic neutrophils. We find that changes in the protrusion rate at the cell front are instantaneously coupled to changes in retraction at the cell rear, while myosin II accumulation at the rear exhibits a reproducible 9-15-s lag. In turning cells, myosin II exhibits dynamic side-to-side relocalization at the cell rear in response to turning of the leading edge and facilitates efficient turning by rapidly re-orienting the rear. These manifestations of front-rear coupling can be explained by a simple quantitative model incorporating reversible actin-myosin interactions with a rearward-flowing actin network. Finally, the system can be tuned by the degree of myosin regulatory light chain (MRLC) phosphorylation, which appears to be set in an optimal range to balance persistence of movement and turning ability.
Author List
Tsai TY, Collins SR, Chan CK, Hadjitheodorou A, Lam PY, Lou SS, Yang HW, Jorgensen J, Ellett F, Irimia D, Davidson MW, Fischer RS, Huttenlocher A, Meyer T, Ferrell JE Jr, Theriot JAAuthor
Pui Ying Lam PhD Assistant Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Actin CytoskeletonActins
Animals
Animals, Genetically Modified
Cell Line
Cell Movement
Cell Polarity
Cell Surface Extensions
Chemotaxis
Cytoskeletal Proteins
Cytoskeleton
Female
Humans
Myosin Type II
Myosins
Neutrophils
Zebrafish
Zebrafish Proteins
