Developing zebrafish disease models for in vivo small molecule screens. Curr Opin Chem Biol 2019 Jun;50:37-44
Date
04/01/2019Pubmed ID
30928773Pubmed Central ID
PMC6800242DOI
10.1016/j.cbpa.2019.02.005Scopus ID
2-s2.0-85063391592 (requires institutional sign-in at Scopus site) 57 CitationsAbstract
The zebrafish is a model organism that allows in vivo studies to be performed at a scale usually restricted to in vitro studies. As such, the zebrafish is well suited to in vivo screens, in which thousands of small molecules are tested for their ability to modify disease phenotypes in zebrafish disease models. Numerous approaches have been developed for modeling human diseases in zebrafish, including mutagenesis, transgenesis, pharmacological approaches, wounding, and exposure to infectious or cancerous agents. We review the various strategies for modeling human diseases in zebrafish and discuss important considerations when developing zebrafish models for use in in vivo small molecule screens.
Author List
Lam PY, Peterson RTAuthor
Pui Ying Lam PhD Assistant Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsDisease Models, Animal
Drug Evaluation, Preclinical
High-Throughput Screening Assays
Humans
Mutagenesis
Regeneration
Wound Healing
Zebrafish
