Live imaging reveals distinct modes of neutrophil and macrophage migration within interstitial tissues. J Cell Sci 2017 Nov 15;130(22):3801-3808
Date
10/04/2017Pubmed ID
28972134Pubmed Central ID
PMC5702045DOI
10.1242/jcs.206128Scopus ID
2-s2.0-85034080622 (requires institutional sign-in at Scopus site) 66 CitationsAbstract
Cell motility is required for diverse processes during immunity and inflammation. Classically, leukocyte motility is defined as an amoeboid type of migration, however some leukocytes, like macrophages, also employ a more mesenchymal mode of migration. Here, we sought to characterize the mechanisms that regulate neutrophil and macrophage migration in vivo by using real-time imaging of leukocyte motility within interstitial tissues in zebrafish larvae. Neutrophils displayed a rounded morphology and rapid protease-independent motility, lacked defined paxillin puncta, and had persistent rearward polarization of stable F-actin and the microtubule network. By contrast, macrophages displayed an elongated morphology with reduced speed and increased directional persistence and formed paxillin-containing puncta but had a less-defined polarization of the microtubule and actin networks. We also observed differential effects of protease inhibition, microtubule disruption and ROCK inhibition on the efficiency of neutrophil and macrophage motility. Taken together, our findings suggest that larval zebrafish neutrophils and macrophage display distinct modes of migration within interstitial tissues in vivo.
Author List
Barros-Becker F, Lam PY, Fisher R, Huttenlocher AAuthor
Pui Ying Lam PhD Assistant Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCell Movement
Cell Polarity
Cell Shape
Cell Tracking
Larva
Macrophages
Microscopy, Fluorescence
Microscopy, Video
Microtubules
Neutrophils
Paxillin
Peptide Hydrolases
Zebrafish
Zebrafish Proteins
