Faculty Collaboration Database

Effect of different femtosecond laser-firing patterns on collagen disruption during refractive lenticule extraction. J Cataract Refract Surg 2012 Aug;38(8):1467-75

Date

07/21/2012

Pubmed ID

22814054

DOI

10.1016/j.jcrs.2012.03.037

Scopus ID

2-s2.0-84864123052 (requires institutional sign-in at Scopus site)   30 Citations

Abstract

PURPOSE: To evaluate the changes in corneal collagen architecture subjected to different laser-firing patterns during refractive lenticule extraction.

SETTING: Singapore Eye Research Institute, Singapore.

DESIGN: Experimental study.

METHODS: Refractive lenticule extraction was performed in rabbits without lenticule removal. Rabbits were divided into 4 groups that had incisions in the following firing patterns: (A) from periphery in (lenticule's posterior surface) and from center out (lenticule's anterior surface); (B) from center out and from center out; (C) from periphery in and from periphery in; and (D) from center out and from periphery in. The corneas were collected 18 hours postoperatively and were subjected to immunofluorescent staining of fibronectin, CD11b, and collagen type I. Ultrastructural analysis was performed using transmission electron microscopy (TEM).

RESULTS: Refractive lenticule extraction-treated corneas showed no significant difference in fibronectin and CD11b expression. Similar expression patterns of collagen type I were observed in corneas that had femtosecond firing patterns A, B, and C; however, a discontinuous and relatively more intense staining pattern along the anterior plane of the lenticule was detected in corneas treated with pattern D. The TEM also showed a more disrupted collagen arrangement along the anterior incision site in pattern D-treated corneas.

CONCLUSIONS: Differential laser firing patterns during refractive lenticule extraction resulted in different levels of collagen derangement along the anterior plane of the lenticule, with pattern D showing the most disrupted surface. Such disruption in the collagen architecture might affect postoperative visual recovery and refractive outcomes.

FINANCIAL DISCLOSURE: No author has a financial or proprietary interest in any material or method mentioned.

Author List

Riau AK, Angunawela RI, Chaurasia SS, Tan DT, Mehta JS

Author

Shyam S. Chaurasia PhD Associate Professor in the Ophthalmology and Visual Sciences department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
CD11b Antigen
Collagen Type I
Corneal Stroma
Corneal Surgery, Laser
Fibronectins
Fluorescent Antibody Technique, Indirect
Lasers, Excimer
Microscopy, Confocal
Microscopy, Electron, Transmission
Rabbits
Surgical Flaps
Tissue Fixation