Faculty Collaboration Database

Complementary and divergent functions of zebrafish Tango1 and Ctage5 in tissue development and homeostasis. Mol Biol Cell 2021 Mar 01;32(5):391-401

Date

01/14/2021

Pubmed ID

33439675

Pubmed Central ID

PMC8098853

DOI

10.1091/mbc.E20-11-0745

Scopus ID

2-s2.0-85101912245 (requires institutional sign-in at Scopus site)   8 Citations

Abstract

Coat protein complex II (COPII) factors mediate cargo export from the endoplasmic reticulum (ER), but bulky collagens and lipoproteins are too large for traditional COPII vesicles. Mammalian CTAGE5 and TANGO1 have been well characterized individually as specialized cargo receptors at the ER that function with COPII coats to facilitate trafficking of bulky cargoes. Here, we present a genetic interaction study in zebrafish of deletions in ctage5, tango1, or both to investigate their distinct and complementary potential functions. We found that Ctage5 and Tango1 have different roles related to organogenesis, collagen versus lipoprotein trafficking, stress-pathway activation, and survival. While disruption of both ctage5 and tango1 compounded phenotype severity, mutation of either factor alone revealed novel tissue-specific defects in the building of heart, muscle, lens, and intestine, in addition to previously described roles in the development of neural and cartilage tissues. Together, our results demonstrate that Ctage5 and Tango1 have overlapping functions, but also suggest divergent roles in tissue development and homeostasis.

Author List

Clark EM, Link BA

Author

Brian A. Link PhD Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Antigens, Neoplasm
Aryl Hydrocarbon Receptor Nuclear Translocator
COP-Coated Vesicles
Carrier Proteins
Endoplasmic Reticulum
Golgi Apparatus
Homeostasis
Neoplasm Proteins
Protein Transport
Vesicular Transport Proteins
Zebrafish
Zebrafish Proteins