Performance of the BD GeneOhm MRSA achromopeptidase assay for real-time PCR detection of methicillin-resistant Staphylococcus aureus in nasal specimens. J Clin Microbiol 2011 Jun;49(6):2266-8
Date
04/22/2011Pubmed ID
21508148Pubmed Central ID
PMC3122740DOI
10.1128/JCM.02431-10Scopus ID
2-s2.0-79957516092 (requires institutional sign-in at Scopus site) 25 CitationsAbstract
We evaluated the BD GeneOhm MRSA achromopeptidase (ACP) assay, which incorporates a new specimen preparation approach. A total of 1,216 leftover nasal samples were tested; using culture as the gold standard, the sensitivity and specificity were 92% and 94.6%, respectively. The new lysis method provides good sensitivity and simplifies specimen preparation.
Author List
Patel PA, Ledeboer NA, Ginocchio CC, Condon S, Bouchard S, Qin P, Karchmer T, Peterson LRAuthor
Nathan A. Ledeboer PhD Vice Chair, Professor in the Pathology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Bacterial ProteinsBacteriological Techniques
Humans
Methicillin-Resistant Staphylococcus aureus
Molecular Diagnostic Techniques
Nasal Mucosa
Polymerase Chain Reaction
Reagent Kits, Diagnostic
Sensitivity and Specificity
Serine Endopeptidases
Specimen Handling
Staphylococcal Infections