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Performance of the BD GeneOhm MRSA achromopeptidase assay for real-time PCR detection of methicillin-resistant Staphylococcus aureus in nasal specimens. J Clin Microbiol 2011 Jun;49(6):2266-8

Date

04/22/2011

Pubmed ID

21508148

Pubmed Central ID

PMC3122740

DOI

10.1128/JCM.02431-10

Scopus ID

2-s2.0-79957516092 (requires institutional sign-in at Scopus site)   25 Citations

Abstract

We evaluated the BD GeneOhm MRSA achromopeptidase (ACP) assay, which incorporates a new specimen preparation approach. A total of 1,216 leftover nasal samples were tested; using culture as the gold standard, the sensitivity and specificity were 92% and 94.6%, respectively. The new lysis method provides good sensitivity and simplifies specimen preparation.

Author List

Patel PA, Ledeboer NA, Ginocchio CC, Condon S, Bouchard S, Qin P, Karchmer T, Peterson LR

Author

Nathan A. Ledeboer PhD Vice Chair, Professor in the Pathology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Bacterial Proteins
Bacteriological Techniques
Humans
Methicillin-Resistant Staphylococcus aureus
Molecular Diagnostic Techniques
Nasal Mucosa
Polymerase Chain Reaction
Reagent Kits, Diagnostic
Sensitivity and Specificity
Serine Endopeptidases
Specimen Handling
Staphylococcal Infections