Rbpj direct regulation of Atoh7 transcription in the embryonic mouse retina. Sci Rep 2018 Jul 05;8(1):10195
Date
07/07/2018Pubmed ID
29977079Pubmed Central ID
PMC6033939DOI
10.1038/s41598-018-28420-yScopus ID
2-s2.0-85049684995 (requires institutional sign-in at Scopus site) 15 CitationsAbstract
In vertebrate retinal progenitor cells, the proneural factor Atoh7 exhibits a dynamic tissue and cellular expression pattern. Although the resulting Atoh7 retinal lineage contains all seven major cell types, only retinal ganglion cells require Atoh7 for proper differentiation. Such specificity necessitates complex regulation of Atoh7 transcription during retina development. The Notch signaling pathway is an evolutionarily conserved suppressor of proneural bHLH factor expression. Previous in vivo mouse genetic studies established the cell autonomous suppression of Atoh7 transcription by Notch1, Rbpj and Hes1. Here we identify four CSL binding sites within the Atoh7 proximal regulatory region and demonstrate Rbpj protein interaction at these sequences by in vitro electromobility shift, calorimetry and luciferase assays and, in vivo via colocalization and chromatin immunoprecipitation. We found that Rbpj simultaneously represses Atoh7 transcription using both Notch-dependent and -independent pathways.
Author List
Miesfeld JB, Moon MS, Riesenberg AN, Contreras AN, Kovall RA, Brown NLAuthor
Joel Bryan Miesfeld PhD Assistant Professor in the Ophthalmology and Visual Sciences department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBasic Helix-Loop-Helix Transcription Factors
Binding Sites
Gene Expression Regulation
Immunoglobulin J Recombination Signal Sequence-Binding Protein
Mice
Nerve Tissue Proteins
Receptors, Notch
Regulatory Elements, Transcriptional
Retina
Signal Transduction
Transcription, Genetic
