S-nitrosoglutathione induces formation of nitrosylmyoglobin in isolated hearts during cardioplegic ischemia--an electron spin resonance study. FEBS Lett 1996 Jan 08;378(2):111-4
Date
01/08/1996Pubmed ID
8549814DOI
10.1016/0014-5793(95)01429-2Scopus ID
2-s2.0-0030060141 (requires institutional sign-in at Scopus site) 23 CitationsAbstract
Previously, it has been shown that *NO donor S-nitrosoglutathione (GSNO) improves the postischemic functional recovery in crystalloid buffer-perfused isolated rat hearts subjected to cardioplegic ischemia. Supplementation of cardioplegic solution with nitronyl nitroxide, a scavenger of *NO, antagonized this protective effect. Using low temperature ESR, we have detected nitrosylmyoglobin (MbNO) in rat hearts subjected to cardioplegic ischemia in the presence of GSNO (20-200 mumol/l). During aerobic reperfusion MbNO signal intensity gradually decreased, but persisted for up to 30 min of aerobic reperfusion. We conclude that MbNO is an endogenous marker of *NO release in myocardial tissues. Implications of MbNO formation are discussed with respect to cardioprotection during ischemia- and reperfusion-induced myocardial injury.
Author List
Konorev EA, Joseph J, Kalyanaraman BAuthor
Balaraman Kalyanaraman PhD Professor in the Biophysics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCardioplegic Solutions
Electron Spin Resonance Spectroscopy
Free Radical Scavengers
Glutathione
Male
Myocardial Ischemia
Myocardial Reperfusion
Myocardium
Myoglobin
Nitric Oxide
Nitroso Compounds
Perfusion
Rats
Rats, Sprague-Dawley
S-Nitrosoglutathione
