Faculty Collaboration Database

Relative expression of insulin receptor isoforms does not differ in lean, obese, and noninsulin-dependent diabetes mellitus subjects. J Clin Endocrinol Metab 1993 May;76(5):1380-2

Date

05/01/1993

Pubmed ID

7684396

DOI

10.1210/jcem.76.5.7684396

Scopus ID

2-s2.0-0027298157 (requires institutional sign-in at Scopus site)   39 Citations

Abstract

The insulin receptor is expressed as two isoforms that differ by a 12-amino acid region at the carboxy-terminus of the alpha-subunit encoded by exon 11. These isoforms are produced by tissue-specific alternate splicing of the insulin receptor mRNA. To determine whether the relative expression of the isoforms is altered in skeletal muscle in two insulin-resistant states, NIDDM and obesity, relative mRNA levels were measured using a polymerase chain reaction technique. There were no differences in the relative amounts of skeletal muscle mRNA encoding the exon 11-containing form compared to the exon 11-lacking form of the insulin receptor among lean normal (30 +/- 2% Ex11-), obese nondiabetic (32 +/- 2%), and NIDDM (31 +/- 1%) subjects. We conclude that altered expression of insulin receptor isoform mRNAs does not account for skeletal muscle insulin resistance in NIDDM and obesity.

Author List

Anderson CM, Henry RR, Knudson PE, Olefsky JM, Webster NJ

Author

Paul Knudson MD Associate Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adult
Aged
Base Sequence
Diabetes Mellitus, Type 2
Exons
Humans
Isomerism
Male
Middle Aged
Molecular Sequence Data
Muscles
Obesity
Oligonucleotide Probes
Polymerase Chain Reaction
RNA
Receptor, Insulin
Reference Values
Transcription, Genetic