Faculty Collaboration Database

Autoantibodies to Abl and Bcr proteins. Leukemia 2000 Sep;14(9):1661-6

Date

09/20/2000

Pubmed ID

10995014

DOI

10.1038/sj.leu.2401870

Scopus ID

2-s2.0-0033835428 (requires institutional sign-in at Scopus site)   3 Citations

Abstract

Formation of an aberrant, chimeric Bcr-Abl protein is the hallmark of Philadelphia (Ph) chromosome-positive leukemias. The Bcr-Abl protein, as well as its normal cellular counterparts--Abl and Bcr--are intracellular molecules with postulated roles in a variety of critical biologic functions. In this study, we demonstrate the existence of autoantibodies against these proteins. Plasma from 18 of 31 individuals (58%), including 14 of 20 Ph-positive CML patients (70%), two of four normal volunteers (50%), and two of seven patients with Ph-negative leukemia (29%) recognized p210Bcr-Abl when used in immunoprecipitation followed by immunoblotting experiments. In all 18 patients, plasma was able to recognize baculovirus-expressed Abl protein; in four patients, recognition of baculovirus-expressed Bcr protein was also demonstrated. These observations suggest that a humoral immune response to p210Bcr-Abl is discernible in both Ph-positive and -negative leukemias and in healthy individuals, and is most likely due to autoantibodies which recognize normal Abl and, to a lesser extent, normal Bcr proteins.

Author List

Talpaz M, Qiu X, Cheng K, Cortes JE, Kantarjian H, Kurzrock R

Author

Razelle Kurzrock MD Center Associate Director, Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adult
Aged
Autoantibodies
Female
Fusion Proteins, bcr-abl
Humans
K562 Cells
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Leukemia, Myeloid, Chronic, Atypical, BCR-ABL Negative
Male
Middle Aged
Oncogene Proteins
Philadelphia Chromosome
Protein-Tyrosine Kinases
Proto-Oncogene Proteins
Proto-Oncogene Proteins c-abl
Proto-Oncogene Proteins c-bcr