Faculty Collaboration Database

The adhesion of clots in wounds contributes to hemostasis and can be enhanced by coagulation factor XIII. Sci Rep 2020 Nov 18;10(1):20116

Date

11/20/2020

Pubmed ID

33208779

Pubmed Central ID

PMC7675984

DOI

10.1038/s41598-020-76782-z

Scopus ID

2-s2.0-85096207089 (requires institutional sign-in at Scopus site)   8 Citations

Abstract

The adhesion of blood clots to wounds is necessary to seal injured vasculature and achieve hemostasis. However, it has not been specifically tested if adhesive failure of clots is a major contributor to rebleeding and what mechanisms prevent clot delamination. Here, we quantified the contribution of adhesive and cohesive failure to rebleeding in a rat model of femoral artery injury, and identified mechanisms that contribute to the adhesive strength of bulk clots in a lap-shear test in vitro. In the rat bleeding model, the frequency of clot failures correlated positively with blood loss (R = 0.81, p = 0.014) and negatively with survival time (R =  - 0.89, p = 0.0030), with adhesive failures accounting for 51 ± 14% of rebleeds. In vitro, adhesion depended on fibrinogen and coagulation factor XIII (FXIII), and supraphysiological FXIII improved adhesive strength. Furthermore, when exogenous FXIII was topically applied into the wound pocket of rats, eleven adhesive failures occurred between eight rats, compared to seventeen adhesive failures between eight untreated rats, whereas the number of cohesive failures remained the same at sixteen in both groups. In conclusion, rebleeding from both adhesive and cohesive failure of clots decreases survival from hemorrhage in vivo. Both endogenous and exogenous FXIII improves the adhesive strength of clots.

Author List

Chan KYT, Yong ASM, Wang X, Ringgold KM, St John AE, Baylis JR, White NJ, Kastrup CJ

Author

Christian Kastrup PhD Professor in the Surgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Administration, Topical
Animals
Blood Platelets
Erythrocytes
Factor XIII
Femoral Artery
Fibrinogen
Hemorrhage
Hemostasis
Humans
Male
Rats, Sprague-Dawley
Thrombosis
Wounds and Injuries