Anti-adenovirus type 5 cytotoxic T lymphocytes: immunodominant epitopes are encoded by the E1A gene. J Virol 1991 Mar;65(3):1450-7
Date
03/01/1991Pubmed ID
1704928Pubmed Central ID
PMC239925DOI
10.1128/JVI.65.3.1450-1457.1991Scopus ID
2-s2.0-0025981970 (requires institutional sign-in at Scopus site) 27 CitationsAbstract
Virus specific, major histocompatibility complex-restricted, cytotoxic T lymphocytes (CTL) generated in Fischer strain rats infected with human adenovirus type 5 (Ad5) were found to recognize antigenic determinants encoded within the Ad5 early region 1A (E1A) gene. Preliminary mapping studies suggest that the E1A CTL epitopes are encoded within the regions between bp 625 to 810 and 916 to 974 in the first exon of this gene. These epitope-coding regions occur within subregions of E1A that are conserved functionally, and to some extent structurally (approximately 50% sequence homology), among adenoviruses of different groups. Nevertheless, Ad5-specific CTL lysed only targets infected with adenoviruses of the same group (group C; e.g., Ad2) and not targets infected with adenoviruses of different groups (groups A, B, and E). These results suggest that virus-specific CTL may limit adenoviral dissemination by destroying virus-infected cells at an early stage in the viral replicative cycle, during E1A gene expression. Expression of other adenovirus genes does not appear to be required to target infected cells for elimination by CTL.
Author List
Routes JM, Bellgrau D, McGrory WJ, Bautista DS, Graham FL, Cook JLAuthor
John M. Routes MD Chief, Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Adenovirus Early ProteinsAdenoviruses, Human
Animals
Antigens, Viral, Tumor
Cell Line
Chromosome Deletion
Cross Reactions
Cytotoxicity, Immunologic
Epitopes
Genes, Viral
Oncogene Proteins, Viral
Rats
Rats, Inbred F344
Restriction Mapping
T-Lymphocytes, Cytotoxic
