Generation of gene-specific mutated rats using zinc-finger nucleases. Methods Mol Biol 2010;597:211-25
Date
12/17/2009Pubmed ID
20013236DOI
10.1007/978-1-60327-389-3_15Scopus ID
2-s2.0-77449157725 (requires institutional sign-in at Scopus site) 92 CitationsAbstract
The genetic dissection of physiological and pathological traits in laboratory model organisms is accelerated by the ability to engineer loss-of-function mutations at investigator-specified loci. This chapter describes the use of zinc-finger nucleases (ZFNs) for the targeted disruption of endogenous rat genes directly in the embryo. ZFNs can specifically disrupt target genes in cultured rat cells and in embryos from inbred and outbred strains, leading to permanently genetically modified animals. This technology allows for the rapid, targeted modification of the rat genome.
Author List
Geurts AM, Cost GJ, Rémy S, Cui X, Tesson L, Usal C, Ménoret S, Jacob HJ, Anegon I, Buelow RAuthor
Aron Geurts PhD Professor in the Physiology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAnimals, Genetically Modified
Cells, Cultured
Deoxyribonucleases, Type II Site-Specific
Embryo Transfer
Female
Gene Knockout Techniques
Genes
Genome
Male
Protein Engineering
RNA, Messenger
Rats
Transcription, Genetic
Zinc Fingers