Method to Study Adaptive NK Cells Following MCMV Infections. Methods Mol Biol 2022;2463:195-204
Date
03/29/2022Pubmed ID
35344176DOI
10.1007/978-1-0716-2160-8_14Scopus ID
2-s2.0-85127680170 (requires institutional sign-in at Scopus site)Abstract
Immunological memory is a fundamental feature of the adaptive immune system that protects the host from recurrent infections from pathogens. Natural killer (NK) cells are a predominant member of the innate immune system that lack clonotypic receptors, which are essential for memory formation. However, evidence demonstrates that a unique subpopulation of NK cells develops adaptive-like features using germline-encoded receptors. Recent studies have shown that infection of cytomegalovirus (CMV) leads to clonal expansion of NKG2C+ and Ly49H+ NK cells, in humans and mouse, respectively. These activation receptors have the capability to recognize CMV-encoded proteins and facilitate a recall response upon reinfection. Although NK cells do not rearrange genes encoding their activating receptors as seen in B and T cells, they possess a selective process to generate memory features and a long-lived progeny. Here, we describe an established in vivo protocol for infecting mice with mouse cytomegalovirus (MCMV) to study an adaptive NK cell response.
Author List
Khalil M, Mei A, Hashemi E, Wang D, Schumacher M, Terhune S, Malarkannan SAuthors
Subramaniam Malarkannan PhD Professor in the Medicine department at Medical College of WisconsinScott Terhune PhD Professor in the Microbiology and Immunology department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AnimalsCytomegalovirus
Cytomegalovirus Infections
Immunologic Memory
Killer Cells, Natural
Mice
Muromegalovirus
