Faculty Collaboration Database

Prognostic factors in resectable pancreatic cancer: p53 and bcl-2. J Gastrointest Surg 1999;3(3):263-77

Date

09/11/1999

Pubmed ID

10481119

DOI

10.1016/s1091-255x(99)80068-7

Scopus ID

2-s2.0-0033122710 (requires institutional sign-in at Scopus site)   45 Citations

Abstract

The p53 tumor suppressor gene and the Bcl-2 proto-oncogene regulate cell cycle progression and apoptosis. We evaluated the expression of these molecular markers with standard pathologic prognostic variables in patients who received multimodality therapy for resectable adenocarcinoma of the pancreas to study the effect of p53 and Bcl-2 on survival duration. Immunohistochemical staining of archival material was performed to determine levels of expression of p53 and Bcl-2 proteins in 70 patients with adenocarcinoma of pancreatic origin. All patients underwent a potentially curative pancreaticoduodenectomy and standardized pathologic analysis of resected specimens. Potential pathologic and molecular prognostic variables were assessed for their effect on survival duration. Nuclear staining for p53 was observed in 33 (47%) of 70 specimens. Immunostaining for Bcl-2 was observed in 23 specimens (33%). A trend toward improved survival duration was seen in patients whose tumors stained positive for either p53 or Bcl-2. Negative staining for both markers predicted short survival (P = 0.01). By univariate and multivariate analyses, no single pathologic factor was associated with survival duration. Immunohistochemical staging using both p53 and Bcl-2 significantly predicted survival duration by univariate and multivariate analysis; patients whose tumors stained positively for p53 and/or overexpressed Bcl-2 had a significantly longer survival than those whose tumors stained negative for both proteins.

Author List

Bold RJ, Hess KR, Pearson AS, Grau AM, Sinicrope FA, Jennings M, McConkey DJ, Bucana CD, Cleary KR, Hallin PA, Chiao PJ, Abbruzzese JL, Evans DB

Author

Douglas B. Evans MD Chair, Professor in the Surgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adenocarcinoma
Analysis of Variance
Apoptosis
Biomarkers, Tumor
Cell Cycle
Cell Nucleus
Coloring Agents
Combined Modality Therapy
Female
Forecasting
Gene Expression Regulation, Neoplastic
Genes, bcl-2
Genes, p53
Humans
Immunohistochemistry
Male
Middle Aged
Multivariate Analysis
Neoplasm Staging
Pancreatic Neoplasms
Pancreaticoduodenectomy
Prognosis
Prospective Studies
Proto-Oncogene Proteins c-bcl-2
Survival Rate
Tumor Suppressor Protein p53