Prevalence of human herpesvirus 6 variant A and B infections in bone marrow transplant recipients as determined by polymerase chain reaction and sequence-specific oligonucleotide probe hybridization. J Clin Microbiol 1993 Jun;31(6):1515-20
Date
06/01/1993Pubmed ID
8391023Pubmed Central ID
PMC265570DOI
10.1128/jcm.31.6.1515-1520.1993Scopus ID
2-s2.0-0027284945 (requires institutional sign-in at Scopus site) 65 CitationsAbstract
An oligotyping methodology was devised by using the polymerase chain reaction and sequence-specific oligonucleotide probe hybridization in order to discriminate the A and B variants of human herpesvirus 6 (HHV-6). Comparative DNA sequence analysis of portions of the U1102 (variant A) and Z29 (variant B) genomes revealed polymorphic regions which allowed for the synthesis of variant-specific and consensus oligonucleotide probes. These probes were found to hybridize exclusively to their respective HHV-6 variants. This strategy was then further tested by evaluating 16 clinical isolates derived from patients undergoing bone marrow transplantation to determine the subtype prevalence of HHV-6 infection in these patients. All clinical isolates were documented to be of variant B, indicating that the majority of bone marrow transplantation patients may be preferentially infected with this HHV-6 subtype. This oligotyping strategy may be useful in defining the relative prevalence of HHV-6A and HHV-6B infections in patient populations potentially at risk for HHV-6 disease.
Author List
Drobyski WR, Eberle M, Majewski D, Baxter-Lowe LAAuthor
William R. Drobyski MD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AdultBase Sequence
Bone Marrow Transplantation
Child
DNA, Viral
Female
Genetic Variation
Herpesviridae Infections
Herpesvirus 6, Human
Humans
Male
Middle Aged
Molecular Sequence Data
Nucleic Acid Hybridization
Oligonucleotide Probes
Polymerase Chain Reaction
Sequence Homology, Nucleic Acid
