Faculty Collaboration Database

Localization of authentic thromboxane A2/prostaglandin H2 receptor in the rat kidney. Kidney Int 1996 May;49(5):1207-13

Date

05/01/1996

Pubmed ID

8731083

DOI

10.1038/ki.1996.174

Scopus ID

2-s2.0-0030007553 (requires institutional sign-in at Scopus site)   25 Citations

Abstract

Using a polyclonal antibody against authentic thromboxane A2/prostaglandin H2 (TxA2/PGH2) receptor protein, we assessed the distribution of this receptor in the normal rat kidney by routine methods of immunofluorescence microscopy. The receptor localized both in glomeruli and in tubules. In the former, the distribution of the receptor was most prominent along the lumen of glomerular capillary loops. Parietal epithelial cells of the Bowman's capsule, podocytes and mesangial cells also demonstrated immunostainable receptor. In the tubules, the receptor localized most prominently at the base of the brush border of proximal tubules and at the luminal surface of thick ascending limbs and distal convoluted tubules. These observations point to sites that are likely to be targeted by thromboxane A2 in forms of renal injury characterized by enhanced synthesis of this eicosanoid.

Author List

Bresnahan BA, Le Breton GC, Lianos EA

Author

Barbara A. Bresnahan MD Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Antibodies
Kidney
Male
Microscopy, Fluorescence
Rats
Rats, Sprague-Dawley
Receptors, Prostaglandin
Receptors, Thromboxane
Receptors, Thromboxane A2, Prostaglandin H2
Tissue Distribution