Faculty Collaboration Database

Analysis of IFT kinesins in developing zebrafish cone photoreceptor sensory cilia. Methods Cell Biol 2009;93:219-34

Date

01/01/2009

Pubmed ID

20409820

DOI

10.1016/S0091-679X(08)93012-0

Scopus ID

2-s2.0-77954674137 (requires institutional sign-in at Scopus site)   8 Citations

Abstract

The photoreceptor outer segment (OS), a well-defined sensory cilium, provides an important context for the study of intraflagellar transport (IFT). The early phases of OS development involve successive events that are common to virtually all cilia. Additionally, intense protein trafficking occurs through the cilium and relies on IFT to maintain proper cellular morphology and optimize the photosensitive function. In the past decade, progress has been made in the characterization of photoreceptor OS trafficking in murine and amphibian models. Recently, powerful and cost-effective molecular tools and techniques for zebrafish have opened new opportunities to study photoreceptor IFT. Studies using zebrafish take advantage of its rapid embryogenesis to characterize the early events involved in photoreceptor ciliogenesis and OS assembly. In this overview, we describe phenotypes associated with knockdown strategies or genetic mutations of IFT components in zebrafish and detail a general experimental approach that has enabled us to study the function of the two anterograde IFT motors, KIF17 and kinesin II, in zebrafish cone photoreceptors.

Author List

Insinna C, Luby-Phelps K, Link BA, Besharse JC

Author

Brian A. Link PhD Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Animals, Genetically Modified
Axoneme
Biological Transport
Cilia
Flagella
Models, Animal
Molecular Motor Proteins
Mutation
Oligonucleotides, Antisense
Phenotype
Promoter Regions, Genetic
Retinal Cone Photoreceptor Cells
Zebrafish