Expression of natural killer receptors in T- and NK-cells: comparison of healthy individuals, patients with prior stem cell transplant, and patients undergoing chemotherapy. Leuk Lymphoma 2010 Mar;51(3):481-7
Date
02/10/2010Pubmed ID
20141431DOI
10.3109/10428190903552120Scopus ID
2-s2.0-79954619318 (requires institutional sign-in at Scopus site) 5 CitationsAbstract
We studied the expression of natural killer receptors (NKRs) on peripheral blood cytotoxic T lymphocytes and NK cells in patients who underwent an allogeneic stem cell transplant (SCT), and compared these findings with results from healthy individuals (CTRL) and patients undergoing chemotherapy (CHEMO), respectively. Peripheral blood mononuclear cells were analyzed by flow cytometry with antibodies against the NKRs CD158a, CD158b, CD158e (known as killer immunoglobulin-like receptors, KIRs), and CD94. Expression of NKRs was evaluated separately in CD56+, CD57+, and CD56/CD57 (double +) subsets of T and NK cells. We found mainly differences in CD158a and CD94 expression between the three cohorts, with the SCT and CHEMO groups usually showing similar changes, when compared to the CTRL population. None of the patients with SCT or CHEMO demonstrated patterns of restricted NKR expression. Our results provide a comprehensive overview of KIR and CD94 expression in T and NK cells following SCT and chemotherapy.
Author List
Olteanu H, Schur BC, Bredeson C, Atallah E, Kroft SHAuthors
Ehab L. Atallah MD Professor in the Medicine department at Medical College of WisconsinSteven Howard Kroft MD Chair, Professor in the Pathology department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AdultAged
Antigens, CD
Antineoplastic Agents
Female
Gene Expression Regulation
Humans
Immunophenotyping
Killer Cells, Natural
Lymphoproliferative Disorders
Male
Middle Aged
Receptors, Natural Killer Cell
Stem Cell Transplantation
T-Lymphocytes, Cytotoxic
Transplantation, Homologous
Young Adult
