Correlation of gene and protein structures in the FXYD family proteins. J Mol Biol 2005 Dec 09;354(4):743-50
Date
11/18/2005Pubmed ID
16288923Pubmed Central ID
PMC2907130DOI
10.1016/j.jmb.2005.10.018Scopus ID
2-s2.0-28444456326 (requires institutional sign-in at Scopus site) 14 CitationsAbstract
The FXYD family proteins are auxiliary subunits of the Na,K-ATPase, expressed primarily in tissues that specialize in fluid or solute transport, or that are electrically excitable. These proteins range in size from about 60 to 160 amino acid residues, and share a core homology of 35 amino acid residues in and around a single transmembrane segment. Despite their relatively small sizes, they are all encoded by genes with six to nine small exons. We show that the helical secondary structures of three FXYD family members, FXYD1, FXYD3, and FXYD4, determined in micelles by NMR spectroscopy, reflect the structures of their corresponding genes. The coincidence of helical regions, and connecting segments, with the positions of intron-exon junctions in the genes, support the hypothesis that the FXYD proteins may have been assembled from discrete structural modules through exon shuffling.
Author List
Franzin CM, Yu J, Thai K, Choi J, Marassi FMAuthor
Francesca M. Marassi PhD Chair, Professor in the Biophysics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsExons
Gene Components
Gene Rearrangement
Humans
Intracellular Signaling Peptides and Proteins
Membrane Proteins
Mice
Micelles
Neoplasm Proteins
Nuclear Magnetic Resonance, Biomolecular
Phosphoproteins
Potassium Channels
Protein Structure, Secondary
Rats
Sodium Dodecyl Sulfate
Sodium-Potassium-Exchanging ATPase
