A simple approach to membrane protein secondary structure and topology based on NMR spectroscopy. Biophys J 2001 Feb;80(2):994-1003
Date
02/13/2001Pubmed ID
11159466Pubmed Central ID
PMC1301297DOI
10.1016/S0006-3495(01)76078-XScopus ID
2-s2.0-0035142956 (requires institutional sign-in at Scopus site) 69 CitationsAbstract
This paper describes a simple, qualitative approach for the determination of membrane protein secondary structure and topology in lipid bilayer membranes. The approach is based on the observation of wheel-like resonance patterns observed in the NMR 1H-15N/15N polarization inversion with spin exchange at the magic angle (PISEMA) and 1H/15N heteronuclear correlation (HETCOR) spectra of membrane proteins in oriented lipid bilayers. These patterns, named Pisa wheels, have been previously shown to reflect helical wheel projections of residues that are characteristic of alpha-helices associated with membranes. This study extends the analysis of these patterns to beta-strands associated with membranes and demonstrates that, as for the case of alpha-helices, Pisa wheels are extremely sensitive to the tilt, rotation, and twist of beta-strands in the membrane. Therefore, the Pisa wheels provide a sensitive, visually accessible, qualitative index of membrane protein secondary structure and topology.
Author List
Marassi FMAuthor
Francesca M. Marassi PhD Chair, Professor in the Biophysics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Biophysical PhenomenaBiophysics
Lipid Bilayers
Magnetic Resonance Spectroscopy
Membrane Proteins
Models, Molecular
Porins
Protein Structure, Secondary
Rhodobacter capsulatus
