Comparative Gene Expression Analyses Reveal Distinct Molecular Signatures between Differentially Reprogrammed Cardiomyocytes. Cell Rep 2017 Sep 26;20(13):3014-3024
Date
09/28/2017Pubmed ID
28954220Pubmed Central ID
PMC5659840DOI
10.1016/j.celrep.2017.09.005Scopus ID
2-s2.0-85029885058 (requires institutional sign-in at Scopus site) 50 CitationsAbstract
Cardiomyocytes derived from induced pluripotent stem cells (iPSC-CMs) or directly reprogrammed from non-myocytes (induced cardiomyocytes [iCMs]) are promising sources for heart regeneration or disease modeling. However, the similarities and differences between iPSC-CMs and iCMs are still unknown. Here, we performed transcriptome analyses of beating iPSC-CMs and iCMs generated from cardiac fibroblasts (CFs) of the same origin. Although both iPSC-CMs and iCMs establish CM-like molecular features globally, iPSC-CMs exhibit a relatively hyperdynamic epigenetic status, whereas iCMs exhibit a maturation status that more closely resembles that of adult CMs. Based on gene expression of metabolic enzymes, iPSC-CMs primarily employ glycolysis, whereas iCMs utilize fatty acid oxidation as the main pathway. Importantly, iPSC-CMs and iCMs exhibit different cell-cycle status, alteration of which influenced their maturation. Therefore, our study provides a foundation for understanding the pros and cons of different reprogramming approaches.
Author List
Zhou Y, Wang L, Liu Z, Alimohamadi S, Yin C, Liu J, Qian LAuthor
Ziqing Liu PhD Assistant Professor in the Physiology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Cell DifferentiationCellular Reprogramming
Gene Expression
Gene Expression Profiling
Humans
Myocytes, Cardiac
