Faculty Collaboration Database

Pertussis toxin abolishes the cardioprotective effect of ischemic preconditioning in intact rat heart. Am J Physiol 1998 Aug;275(2):H495-500

Date

07/31/1998

Pubmed ID

9683437

DOI

10.1152/ajpheart.1998.275.2.H495

Scopus ID

2-s2.0-0031852654 (requires institutional sign-in at Scopus site)   48 Citations

Abstract

It has been previously demonstrated that Gi/o proteins are involved in ischemic preconditioning (IPC) in rabbits and dogs; however, there has been controversy as to the role of Gi/o proteins in IPC in in vivo rat infarct models. Therefore, the role of G proteins in the cardioprotective effect of IPC in a rat infarct model was reevaluated. Cardioprotection as indicated by infarct size (IS) as a percentage of the area at risk (IS/AAR) was determined by triphenyltetrazolium stain. The control group, which was subjected to 30 min of occlusion (Occ) and 2 h of reperfusion (Rep), had an IS/AAR of 46 +/- 6%. A single 5-min Occ followed by 10 min of Rep (1x PC) as well as three 5-min Occ periods interspersed with 5 min of Rep (3x PC) markedly reduced IS/AAR (6 +/- 1 and 8 +/- 1%, respectively). Pretreatment with pertussis toxin (10 microg/kg ip) for 48 h before 1x PC or 3x PC completely abolished their cardioprotective effects (46 +/- 5 and 38 +/- 4%, respectively). Pertussis toxin had no effect on IS/AAR and did not inactivate Gi/o proteins as assessed by an in vitro ADP-ribosylation assay of heart homogenates. These results demonstrate that the cardioprotective effect of IPC is mediated by a small subpopulation of Gi/o proteins in the intact rat heart.

Author List

Schultz JE, Hsu AK, Barbieri JT, Li PL, Gross GJ

Author

Joseph T. Barbieri PhD Professor in the Microbiology and Immunology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Acetylcholine
Adenosine
Adenosine Diphosphate Ribose
Animals
Dogs
GTP-Binding Proteins
Heart Rate
Ischemic Preconditioning, Myocardial
Male
Myocardial Infarction
Myocardial Ischemia
Myocardial Reperfusion Injury
NAD
Pertussis Toxin
Rabbits
Rats
Rats, Wistar
Virulence Factors, Bordetella