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Localization of ASH1 mRNA particles in living yeast. Mol Cell 1998 Oct;2(4):437-45



Pubmed ID




Scopus ID

2-s2.0-0032185810 (requires institutional sign-in at Scopus site)   1224 Citations


ASH1 mRNA localizes to the bud tip in Saccharomyces cerevisiae to establish asymmetry of HO expression, important for mating type switching. To visualize real time localization of the mRNA in living yeast cells, green fluorescent protein (GFP) was fused to the RNA-binding protein MS2 to follow a reporter mRNA containing MS2-binding sites. Formation and localization of a GFP particle in the bud required ASH1 3'UTR (untranslated region) sequences. The SHE mutants disrupt RNA and particle localization and SHE 2 and 3 mutants inhibit particle formation as well. Both She3myc and She1myc colocalized with the particle. Video microscopy demonstrated that She1p/Myo4p moved particles to the bud tip at 200-440 nm/sec. Therefore, the ASH1 3'UTR-dependent particle serves as a marker for RNA transport and localization.

Author List

Bertrand E, Chartrand P, Schaefer M, Shenoy SM, Singer RH, Long RM


Roy M. Long PhD Assistant Dean, Associate Professor in the Medical School Regional Campuses department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

3' Untranslated Regions
Base Sequence
Biological Transport
DNA-Binding Proteins
Fungal Proteins
Green Fluorescent Proteins
Indicators and Reagents
Luminescent Proteins
Microscopy, Fluorescence
Molecular Motor Proteins
Molecular Sequence Data
Myosin Heavy Chains
Myosin Type V
RNA, Fungal
RNA, Messenger
Repressor Proteins
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Transcription Factors
Zinc Fingers