Faculty Collaboration Database

Localization of ASH1 mRNA particles in living yeast. Mol Cell 1998 Oct;2(4):437-45

Date

11/11/1998

Pubmed ID

9809065

DOI

10.1016/s1097-2765(00)80143-4

Scopus ID

2-s2.0-0032185810 (requires institutional sign-in at Scopus site)   1253 Citations

Abstract

ASH1 mRNA localizes to the bud tip in Saccharomyces cerevisiae to establish asymmetry of HO expression, important for mating type switching. To visualize real time localization of the mRNA in living yeast cells, green fluorescent protein (GFP) was fused to the RNA-binding protein MS2 to follow a reporter mRNA containing MS2-binding sites. Formation and localization of a GFP particle in the bud required ASH1 3'UTR (untranslated region) sequences. The SHE mutants disrupt RNA and particle localization and SHE 2 and 3 mutants inhibit particle formation as well. Both She3myc and She1myc colocalized with the particle. Video microscopy demonstrated that She1p/Myo4p moved particles to the bud tip at 200-440 nm/sec. Therefore, the ASH1 3'UTR-dependent particle serves as a marker for RNA transport and localization.

Author List

Bertrand E, Chartrand P, Schaefer M, Shenoy SM, Singer RH, Long RM

Author

Roy M. Long PhD Assistant Dean, Professor in the Medical School Regional Campuses department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

3' Untranslated Regions
Base Sequence
Biological Transport
DNA-Binding Proteins
Fungal Proteins
Green Fluorescent Proteins
Indicators and Reagents
Luminescent Proteins
Microscopy, Fluorescence
Molecular Motor Proteins
Molecular Sequence Data
Mutagenesis
Myosin Heavy Chains
Myosin Type V
Myosins
Plasmids
RNA, Fungal
RNA, Messenger
Repressor Proteins
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Transcription Factors
Zinc Fingers