Expression of mPer1 and mPer2, two mammalian clock genes, in murine bone marrow. Biochem Biophys Res Commun 2000 Sep 24;276(2):724-8
Date
10/12/2000Pubmed ID
11027538DOI
10.1006/bbrc.2000.3536Scopus ID
2-s2.0-0034710791 (requires institutional sign-in at Scopus site) 42 CitationsAbstract
Although circadian variations in hematopoiesis have been well documented, the molecular mechanism of the circadian rhythms remains elusive. To determine if a clock system exists in bone marrow to mediate the circadian rhythms, we analyzed the expression of mPer1 and mPer2, both mouse homologues of the Drosophila period gene and known regulators of the clock system, in murine bone marrow by relative quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). We demonstrated that both genes were expressed in bone marrow. Furthermore, the expression patterns of mPer1 and mPer2 in total bone marrow cells exhibited two peaks over a 24-h period. In contrast, the expression patterns of these two genes in the Gr-1-positive cells isolated from bone marrow mainly contributed to one of the two peaks. These results indicate that a clock system exists in bone marrow and suggest that the circadian rhythms in bone marrow are lineage- and/or differentiation stage-dependent.
Author List
Chen YG, Mantalaris A, Bourne P, Keng P, Wu JHAuthor
Yi-Guang Chen PhD Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Analysis of VarianceAnimals
Biological Clocks
Bone Marrow
Cell Cycle Proteins
Circadian Rhythm
Female
Gene Expression Regulation
Male
Mice
Mice, Inbred BALB C
Nuclear Proteins
Period Circadian Proteins
Transcription Factors
