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Expression of mPer1 and mPer2, two mammalian clock genes, in murine bone marrow. Biochem Biophys Res Commun 2000 Sep 24;276(2):724-8



Pubmed ID




Scopus ID

2-s2.0-0034710791 (requires institutional sign-in at Scopus site)   42 Citations


Although circadian variations in hematopoiesis have been well documented, the molecular mechanism of the circadian rhythms remains elusive. To determine if a clock system exists in bone marrow to mediate the circadian rhythms, we analyzed the expression of mPer1 and mPer2, both mouse homologues of the Drosophila period gene and known regulators of the clock system, in murine bone marrow by relative quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). We demonstrated that both genes were expressed in bone marrow. Furthermore, the expression patterns of mPer1 and mPer2 in total bone marrow cells exhibited two peaks over a 24-h period. In contrast, the expression patterns of these two genes in the Gr-1-positive cells isolated from bone marrow mainly contributed to one of the two peaks. These results indicate that a clock system exists in bone marrow and suggest that the circadian rhythms in bone marrow are lineage- and/or differentiation stage-dependent.

Author List

Chen YG, Mantalaris A, Bourne P, Keng P, Wu JH


Yi-Guang Chen PhD Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Analysis of Variance
Biological Clocks
Bone Marrow
Cell Cycle Proteins
Circadian Rhythm
Gene Expression Regulation
Mice, Inbred BALB C
Nuclear Proteins
Period Circadian Proteins
Transcription Factors