Faculty Collaboration Database

Analysis of continuous enzyme kinetic data using ICEKAT. Methods Enzymol 2023;690:109-129

Date

10/20/2023

Pubmed ID

37858527

Pubmed Central ID

PMC10691744

DOI

10.1016/bs.mie.2023.06.019

Scopus ID

2-s2.0-85166356880 (requires institutional sign-in at Scopus site)

Abstract

ICEKAT (Interactive Continuous Enzyme Analysis Tool) is an interactive web-based program for calculating initial rates and kinetic parameters (e.g., V_max, k_cat, K_M, EC₅₀, IC₅₀) from continuous enzyme kinetic assay data that satisfy Michaelis-Menten and steady-state kinetic assumptions. ICEKAT is valuable in educational and research settings to consistently and accurately calculate initial rates and kinetic parameters, increasing assay veracity and reproducibility. Provided freely online to the scientific community, ICEKAT has been cited in at least 26 publications, and the initial journal article has been accessed nearly 9000 times since its debut in 2020 (Olp et al., 2020). Here, we provide in-depth instructions for software use, offer vital considerations for data analysis, and highlight updated software features for new and existing users. Through ICEKAT, we aim for the analysis of data from continuous enzyme kinetic studies worldwide to become more rapid, reliable, and repeatable. ICEKAT remains free of charge and available to all scientists at https://icekat.herokuapp.com/icekat; the source code for local use is found at https://github.com/SmithLabMCW/icekat.

Author List

Bursch KL, Olp MD, Smith BC

Author

Brian C. Smith PhD Associate Professor in the Biochemistry department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Enzyme Assays
Kinetics
Reproducibility of Results
Software