Zinc-finger nucleases: new strategies to target the rat genome. Clin Sci (Lond) 2010 Jul 06;119(8):303-11
Date
07/10/2010Pubmed ID
20615201Pubmed Central ID
PMC3549593DOI
10.1042/CS20100201Scopus ID
2-s2.0-77956367668 31 CitationsAbstract
The importance of genetic laboratory models, such as mice and rats, becomes evident when there is a poor understanding of the nature of human disease. Many rat models for human disease, created over the years by phenotype-driven strategies, now provide a foundation for the identification of their genetic determinants. These models are especially valuable with the emerging need for validation of genes found in genome-wide association studies for complex diseases. The manipulation of the rat genome using engineered zinc-finger nucleases now introduces a key technology for manipulating the rat genome, which is broadly applicable. The ability to generate knockout rat models using zinc-finger nuclease technology will now enable its full emergence as an exceptional physiological and genetic research model.
Author List
Geurts AM, Moreno CAuthor
Aron Geurts PhD Professor in the Physiology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsDNA Repair
Deoxyribonucleases
Disease Models, Animal
Gene Knockout Techniques
Gene Targeting
Genetic Predisposition to Disease
Genome
Humans
Rats
Zinc Fingers
