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Zinc-finger nucleases: new strategies to target the rat genome. Clin Sci (Lond) 2010 Jul 06;119(8):303-11

Date

07/10/2010

Pubmed ID

20615201

Pubmed Central ID

PMC3549593

DOI

10.1042/CS20100201

Scopus ID

2-s2.0-77956367668   31 Citations

Abstract

The importance of genetic laboratory models, such as mice and rats, becomes evident when there is a poor understanding of the nature of human disease. Many rat models for human disease, created over the years by phenotype-driven strategies, now provide a foundation for the identification of their genetic determinants. These models are especially valuable with the emerging need for validation of genes found in genome-wide association studies for complex diseases. The manipulation of the rat genome using engineered zinc-finger nucleases now introduces a key technology for manipulating the rat genome, which is broadly applicable. The ability to generate knockout rat models using zinc-finger nuclease technology will now enable its full emergence as an exceptional physiological and genetic research model.

Author List

Geurts AM, Moreno C

Author

Aron Geurts PhD Professor in the Physiology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
DNA Repair
Deoxyribonucleases
Disease Models, Animal
Gene Knockout Techniques
Gene Targeting
Genetic Predisposition to Disease
Genome
Humans
Rats
Zinc Fingers