Faculty Collaboration Database

Expression analysis of long non-coding RNAs and their target genes in multiple sclerosis patients. Neurol Sci 2019 Apr;40(4):801-811

Date

01/27/2019

Pubmed ID

30680474

DOI

10.1007/s10072-019-3720-3

Scopus ID

2-s2.0-85060671054 (requires institutional sign-in at Scopus site)   11 Citations

Abstract

Multiple sclerosis (MS) is a progressive chronic autoimmune-mediated disease. Recently, long non-coding RNAs (lncRNAs) are characterized to participate in the adjustment of immune responses. Here, we evaluated the expression levels of GSTT1-AS1 and IFNG-AS1 lncRNAs and their targets (TNF and IFNG, respectively) in Iranian MS patients.In this case-control study, 50 relapsing-remitting MS patients and 50 healthy subjects were recruited. Expressions of GSTT1-AS1 and IFNG-AS1 lncRNAs, as well as TNF and IFNG genes, were assessed in their peripheral blood samples by SYBR Green-based Real-time quantitative PCR.Expression levels of GSTT1-AS1 and IFNG-AS1 lncRNAs were both significantly downregulated (p values 0.032 and 0.013, respectively). On the other hand, the expression of TNF and IFNG showed increased levels, however, did not reach statistical significance after our analysis (p > 0.05). Spearman correlation analysis showed that GSTT1-AS1 had a significant positive moderate correlation with IFNG-AS1 (r = 0.541, p < 0.0001), IFNG (r = 0.329, p = 0.001), and TNF (r = 0.204, p = 0.041). Also, IFNG-AS1 revealed the same correlation with IFNG (r = 0.475, p < 0.0001) as well as TNF (r = 0.399, p < 0.0001). Furthermore, GSTT1-AS1 (r = 0.313, p = 0.027) and (IFNG r = 0.478, p < 0.0001) demonstrated a significant positive correlation with age at onset.Briefly, the current study provided for the first time dysregulation of GSTT1-AS1 and IFNG-AS lncRNAs network in MS, which highlights the significant role of epigenetic pathways in this autoimmune disorder. Larger sample size and further investigation assays could shed light on the underlying mechanisms in this area of science.

Author List

Ganji M, Sayad A, Omrani MD, Arsang-Jang S, Mazdeh M, Taheri M

Author

Shahram Arsang-Jang Postdoctoral Fellow in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adolescent
Adult
Case-Control Studies
Female
Glutathione Transferase
Humans
Interferon-gamma
Iran
Male
Middle Aged
Multiple Sclerosis, Relapsing-Remitting
RNA, Long Noncoding
Signaling Lymphocytic Activation Molecule Family
Tumor Necrosis Factor-alpha
Young Adult