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Cloning, expression, and in vitro activity of human endostatin. Biochem Biophys Res Commun 1999 May 10;258(2):345-52

Date

05/18/1999

Pubmed ID

10329390

DOI

10.1006/bbrc.1999.0595

Abstract

Endostatin, a 20 kDa C-terminal fragment of collagen XVIII, is a specific inhibitor of endothelial cell proliferation and angiogenesis. In the present study, we have expressed human endostatin in a yeast expression system (10 mg/L). The recombinant protein was expressed in a soluble form and purified to homogeneity. It specifically inhibited the proliferation and migration of endothelial cells. In addition, we report for the first time that endostatin caused G1 arrest of endothelial cells. Also, we show that endostatin treatment resulted in apoptosis of HUVE and HMVE cells and that all of these effects do not occur in nonendothelial cells. Collectively, these findings demonstrate the expression of a biologically active form of human endostatin in yeast and provide important mechanistic insight into endostatin action on endothelial cells.

Author List

Dhanabal M, Volk R, Ramchandran R, Simons M, Sukhatme VP

Author

Ramani Ramchandran PhD Professor in the Pediatrics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Annexin A5
Base Sequence
Blotting, Western
Cattle
Cell Cycle
Cell Division
Cell Line
Cell Movement
Cloning, Molecular
Collagen
Collagen Type XVIII
DNA Primers
Endostatins
Endothelium, Vascular
Humans
Peptide Fragments
Pichia
Recombinant Proteins
jenkins-FCD Prod-484 8aa07fc50b7f6d102f3dda2f4c7056ff84294d1d