Cloning, expression, and in vitro activity of human endostatin. Biochem Biophys Res Commun 1999 May 10;258(2):345-52
Date
05/18/1999Pubmed ID
10329390DOI
10.1006/bbrc.1999.0595Scopus ID
2-s2.0-0033542129 (requires institutional sign-in at Scopus site) 199 CitationsAbstract
Endostatin, a 20 kDa C-terminal fragment of collagen XVIII, is a specific inhibitor of endothelial cell proliferation and angiogenesis. In the present study, we have expressed human endostatin in a yeast expression system (10 mg/L). The recombinant protein was expressed in a soluble form and purified to homogeneity. It specifically inhibited the proliferation and migration of endothelial cells. In addition, we report for the first time that endostatin caused G1 arrest of endothelial cells. Also, we show that endostatin treatment resulted in apoptosis of HUVE and HMVE cells and that all of these effects do not occur in nonendothelial cells. Collectively, these findings demonstrate the expression of a biologically active form of human endostatin in yeast and provide important mechanistic insight into endostatin action on endothelial cells.
Author List
Dhanabal M, Volk R, Ramchandran R, Simons M, Sukhatme VPAuthor
Ramani Ramchandran PhD Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAnnexin A5
Base Sequence
Blotting, Western
Cattle
Cell Cycle
Cell Division
Cell Line
Cell Movement
Cloning, Molecular
Collagen
Collagen Type XVIII
DNA Primers
Endostatins
Endothelium, Vascular
Humans
Peptide Fragments
Pichia
Recombinant Proteins