Faculty Collaboration Database

Fabry Disease Rat Model Develops Age- and Sex-Dependent Anterior Segment Ocular Abnormalities. Invest Ophthalmol Vis Sci 2024 Aug 01;65(10):14

Date

08/07/2024

Pubmed ID

39110587

Pubmed Central ID

PMC11314710

DOI

10.1167/iovs.65.10.14

Scopus ID

2-s2.0-85200939798 (requires institutional sign-in at Scopus site)

Abstract

PURPOSE: Fabry disease is an X-linked lysosomal storage disorder that results in multi-systemic renal, cardiovascular, and neuropathological damage, including in the eyes. We evaluated anterior segment ocular abnormalities based on age, sex (male and female), and genotype (wild-type, knockout [KO] male, heterozygous [HET] female, and KO female) in a rat model of Fabry disease.

METHODS: The α-Gal A KO and WT rats were divided into young (6-24 weeks), adult (25-60 weeks), and aged (61+ weeks) groups. Intraocular pressure (IOP) was measured. Eyes were clinically scored for corneal and lens opacity as well as evaluated for corneal epithelial integrity and tear break-up time (TBUT). Anterior chamber depth (ACD) and central corneal thickness (CCT) using anterior segment-optical coherence tomography (AS-OCT).

RESULTS: The Fabry rats showed an age-dependent increase in IOP, predominantly in the male genotype. TBUT was decreased in both male and female groups with aging. Epithelial integrity was defective in KO males and HET females with age. However, it was highly compromised in KO females irrespective of age. Corneal and lens opacities were severely affected irrespective of sex or genotype in the aging Fabry rats. AS-OCT quantification of CCT and ACD also demonstrated age-dependent increases but were more pronounced in Fabry versus WT genotypes.

CONCLUSIONS: Epithelial integrity, corneal, and lens opacities worsened in Fabry rats, whereas IOP and TBUT changes were age-dependent. Similarly, CCT and ACD were age-related but more pronounced in Fabry rats, providing newer insights into the anterior segment ocular abnormalities with age, sex, and genotype in a rat model of Fabry disease.

Author List

Erdman ME, Ch S, Mohiuddin A, Al-Kirwi K, Rasper MR, Sokupa S, Low SWY, Skumatz CMB, De Stefano V, Kassem IS, Chaurasia SS

Authors

Shyam S. Chaurasia PhD Associate Professor in the Ophthalmology and Visual Sciences department at Medical College of Wisconsin
Vinicius Silbiger De Stefano MD, PhD Assistant Professor in the Ophthalmology and Visual Sciences department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Aging
Animals
Anterior Eye Segment
Disease Models, Animal
Eye Abnormalities
Fabry Disease
Female
Intraocular Pressure
Male
Rats
Sex Factors
Tomography, Optical Coherence
alpha-Galactosidase