Dynamic multispectral NIR/SWIR for in vivo lymphovascular architectural and functional quantification. J Biomed Opt 2024 Oct;29(10):106001
Date
09/30/2024Pubmed ID
39347012Pubmed Central ID
PMC11425400DOI
10.1117/1.JBO.29.10.106001Scopus ID
2-s2.0-85205336601 (requires institutional sign-in at Scopus site)Abstract
SIGNIFICANCE: Although the lymphatic system is the second largest circulatory system in the body, there are limited techniques available for characterizing lymphatic vessel function. We report shortwave-infrared (SWIR) imaging for minimally invasive in vivo quantification of lymphatic circulation with superior contrast and resolution compared with near-infrared first window imaging.
AIM: We aim to study the lymphatic structure and function in vivo via SWIR fluorescence imaging.
APPROACH: We evaluated subsurface lymphatic circulation in healthy, adult immunocompromised salt-sensitive Sprague-Dawley rats using two fluorescence imaging modalities: near-infrared first window (NIR-I, 700 to 900 nm) and SWIR (900 to 1800 nm) imaging. We also compared two fluorescent imaging probes: indocyanine green (ICG) and silver sulfide quantum dots (QDs) as SWIR lymphatic contrast agents following intradermal footpad delivery in these rats.
RESULTS: SWIR imaging exhibits reduced scattering and autofluorescence background relative to NIR-I imaging. SWIR imaging with ICG provides 1.7 times better resolution and sensitivity than NIR-I, and SWIR imaging with QDs provides nearly two times better resolution and sensitivity with enhanced vessel distinguishability. SWIR images thus provide a more accurate estimation of in vivo vessel size than conventional NIR-I images.
CONCLUSIONS: SWIR imaging of silver sulfide QDs into the intradermal footpad injection provides superior image resolution compared with conventional imaging techniques using NIR-I imaging with ICG dye.
Author List
Hansen C, Jagtap J, Parchur A, Sharma G, Shafiee S, Sinha S, Himburg H, Joshi AAuthors
Heather A. Himburg PhD Associate Professor in the Radiation Oncology department at Medical College of WisconsinAmit Joshi PhD Professor in the Biomedical Engineering department at Medical College of Wisconsin
Abdul Kareem Parchur Medical Physicist Assistant in the Radiation Oncology department at Medical College of Wisconsin
Shayan Shafiee Postdoctoral Researcher in the Biomedical Engineering department at Medical College of Wisconsin
Sayantan Sinha PhD Postdoctoral Researcher in the Biomedical Engineering department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AnimalsContrast Media
Fluorescent Dyes
Indocyanine Green
Lymphatic Vessels
Optical Imaging
Quantum Dots
Rats
Rats, Sprague-Dawley
Spectroscopy, Near-Infrared
