Faculty Collaboration Database

Effects of CB(1) cannabinoid receptor activation on cerebellar granule cell nitric oxide synthase activity. FEBS Lett 1999 Oct 08;459(2):277-81

Date

10/13/1999

Pubmed ID

10518035

DOI

10.1016/s0014-5793(99)01253-3

Scopus ID

2-s2.0-0032830327 (requires institutional sign-in at Scopus site)   92 Citations

Abstract

Cerebellar granule cells (CGCs) express the CB(1) subtype of cannabinoid receptor. CB(1) receptor agonists Win 55212-2, CP55940 and HU210 inhibit KCl-induced activation of nitric oxide synthase (NOS) in CGCs. Win 55212-2 has no effect on either basal NOS activity or on activation by N-methyl-D-aspartate and its effect is abolished by pre-treatment of the cells with pertussis toxin. The CB(1) receptor antagonist/inverse agonist SR141716A both reverses the effects of Win 55212-2 and produces an increase in NOS activity that is additive with KCl. These results support the hypothesis that activation of the CB(1) receptor in CGCs results in a decreased influx of calcium in response to membrane depolarization, resulting in a decreased activation of neuronal NOS.

Author List

Hillard CJ, Muthian S, Kearn CS

Author

Cecilia J. Hillard PhD Associate Dean, Center Director, Professor in the Pharmacology and Toxicology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Benzoxazines
Calcium Channel Blockers
Cells, Cultured
Cerebellum
Drug Interactions
Female
Male
Morpholines
Naphthalenes
Nitric Oxide Synthase
Nitric Oxide Synthase Type I
Pertussis Toxin
Piperidines
Pyrazoles
Rats
Receptors, Cannabinoid
Receptors, Drug
Virulence Factors, Bordetella