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She2p is a novel RNA-binding protein that recruits the Myo4p-She3p complex to ASH1 mRNA. EMBO J 2000 Dec 01;19(23):6592-601

Date

12/02/2000

Pubmed ID

11101531

Pubmed Central ID

PMC305871

DOI

10.1093/emboj/19.23.6592

Scopus ID

2-s2.0-0034406803   163 Citations

Abstract

In Saccharomyces cerevisiae, Ash1p is a specific repressor of transcription that localizes exclusively to daughter cell nuclei through the asymmetric localization of ASH1 mRNA. This localization requires four cis-acting localization elements located in the ASH1 mRNA, five trans-acting factors, one of which is a myosin, and the actin cytoskeleton. The RNA-binding proteins that interact with these cis-elements remained to be identified. Starting with the 3' most localization element of ASH1 mRNA in the three-hybrid assay, element E3, we isolated a clone corresponding to the C-terminus of She3p. We also found that She3p and She2p interact, and this interaction is essential for the binding of She3p with element E3 in vivo. Moreover, She2p was observed to bind the E3 RNA directly in vitro and each of the ASH1 cis-acting localization elements requires She2p for their localization function. By tethering a She3p-MS2 fusion protein to a reporter RNA containing MS2 binding sites, we observed that She2p is dispensable for She3p-MS2-dependent RNA localization.

Author List

Long RM, Gu W, Lorimer E, Singer RH, Chartrand P

Author

Roy M. Long PhD Assistant Dean, Associate Professor in the Medical School Regional Campuses department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Actins
Cell Nucleus
Cytoskeleton
DNA-Binding Proteins
Escherichia coli
Fungal Proteins
In Situ Hybridization
Lac Operon
Models, Biological
Myosin Heavy Chains
Myosin Type V
Myosins
Plasmids
Protein Binding
RNA, Messenger
RNA-Binding Proteins
Recombinant Fusion Proteins
Repressor Proteins
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Transcription Factors
Transcription, Genetic
Transcriptional Activation
Two-Hybrid System Techniques
Ultraviolet Rays