Dynamic association of gammaherpesvirus DNA with core histone during de novo lytic infection of primary cells. Virology 2011 Dec 20;421(2):167-72
Date
10/25/2011Pubmed ID
22018782Pubmed Central ID
PMC3210866DOI
10.1016/j.virol.2011.09.024Scopus ID
2-s2.0-80355146848 (requires institutional sign-in at Scopus site) 14 CitationsAbstract
Association of herpesvirus DNA with histones has important implications for lytic and latent infections; thus herpesviruses arbitrate interactions with histones to productively infect host cells. While regulation of alpha and betaherpesvirus chromatin during lytic infection has been actively investigated, very little is known about interaction of gammaherpesvirus DNA with histones upon de novo lytic infection. Murine gammaherpesvirus-68 (MHV68) is a rodent pathogen that offers a tractable system to study gammaherpesvirus lytic infection in primary cells. In this study we report that MHV68 promoter and orilyt sequences underwent dynamic association with histone H3 during de novo lytic infection of primary macrophages and fibroblasts. Similar to HSV-1, the degree of MHV68 DNA association with histone H3 was dependent on the multiplicity of infection and was further regulated by viral DNA synthesis. Our work sets a precedent for future studies of gammaherpesvirus chromatin during de novo lytic infection.
Author List
Mounce BC, Tsan FC, Kohler S, Cirillo LA, Tarakanova VLAuthors
Lisa A. Cirillo PhD Assistant Dean, Associate Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of WisconsinVera Tarakanova PhD Professor in the Microbiology and Immunology department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AnimalsCells, Cultured
Chromatin
DNA, Viral
Fibroblasts
Gene Expression Regulation, Viral
Histones
Macrophages
Mice
Mice, Inbred C57BL
Promoter Regions, Genetic
Rhadinovirus
Viral Proteins