Snrk-1 is involved in multiple steps of angioblast development and acts via notch signaling pathway in artery-vein specification in vertebrates. Blood 2009 Jan 29;113(5):1192-9
Date
08/30/2008Pubmed ID
18723694Pubmed Central ID
PMC2635085DOI
10.1182/blood-2008-06-162156Scopus ID
2-s2.0-59649109527 (requires institutional sign-in at Scopus site) 29 CitationsAbstract
In vertebrates, molecular mechanisms dictate angioblasts' migration and subsequent differentiation into arteries and veins. In this study, we used a microarray screen to identify a novel member of the sucrose nonfermenting related kinase (snrk-1) family of serine/threonine kinases expressed specifically in the embryonic zebrafish vasculature and investigated its function in vivo. Using gain- and loss-of-function studies in vivo, we show that Snrk-1 plays an essential role in the migration, maintenance, and differentiation of angioblasts. The kinase function of Snrk-1 is critical for migration and maintenance, but not for the differentiation of angioblasts. In vitro, snrk-1 knockdown endothelial cells show only defects in migration. The snrk-1 gene acts downstream or parallel to notch and upstream of gridlock during artery-vein specification, and the human gene compensates for zebrafish snrk-1 knockdown, suggesting evolutionary conservation of function.
Author List
Chun CZ, Kaur S, Samant GV, Wang L, Pramanik K, Garnaas MK, Li K, Field L, Mukhopadhyay D, Ramchandran RAuthors
Keguo Li MD, PhD Research Scientist I in the Surgery department at Medical College of WisconsinRamani Ramchandran PhD Professor in the Pediatrics department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AnimalsArteries
Basic Helix-Loop-Helix Transcription Factors
Cell Movement
Endothelial Cells
Gene Expression Regulation, Developmental
Gene Expression Regulation, Enzymologic
Gene Knockdown Techniques
Humans
Oligonucleotide Array Sequence Analysis
Receptors, Notch
Veins
Zebrafish
Zebrafish Proteins
