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Snrk-1 is involved in multiple steps of angioblast development and acts via notch signaling pathway in artery-vein specification in vertebrates. Blood 2009 Jan 29;113(5):1192-9

Date

08/30/2008

Pubmed ID

18723694

Pubmed Central ID

PMC2635085

DOI

10.1182/blood-2008-06-162156

Scopus ID

2-s2.0-59649109527   22 Citations

Abstract

In vertebrates, molecular mechanisms dictate angioblasts' migration and subsequent differentiation into arteries and veins. In this study, we used a microarray screen to identify a novel member of the sucrose nonfermenting related kinase (snrk-1) family of serine/threonine kinases expressed specifically in the embryonic zebrafish vasculature and investigated its function in vivo. Using gain- and loss-of-function studies in vivo, we show that Snrk-1 plays an essential role in the migration, maintenance, and differentiation of angioblasts. The kinase function of Snrk-1 is critical for migration and maintenance, but not for the differentiation of angioblasts. In vitro, snrk-1 knockdown endothelial cells show only defects in migration. The snrk-1 gene acts downstream or parallel to notch and upstream of gridlock during artery-vein specification, and the human gene compensates for zebrafish snrk-1 knockdown, suggesting evolutionary conservation of function.

Author List

Chun CZ, Kaur S, Samant GV, Wang L, Pramanik K, Garnaas MK, Li K, Field L, Mukhopadhyay D, Ramchandran R

Authors

Ramani Ramchandran PhD Professor in the Pediatrics department at Medical College of Wisconsin
Ling Wang MD, PhD Assistant Professor in the Obstetrics and Gynecology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Arteries
Basic Helix-Loop-Helix Transcription Factors
Cell Movement
Endothelial Cells
Gene Expression Regulation, Developmental
Gene Expression Regulation, Enzymologic
Gene Knockdown Techniques
Humans
Oligonucleotide Array Sequence Analysis
Protein-Serine-Threonine Kinases
Receptors, Notch
Veins
Zebrafish
Zebrafish Proteins
jenkins-FCD Prod-484 8aa07fc50b7f6d102f3dda2f4c7056ff84294d1d