Quantitative analysis of the immune response to mouse non-MHC transplantation antigens in vivo: the H60 histocompatibility antigen dominates over all others. J Immunol 2001 Apr 01;166(7):4370-9
Date
03/20/2001Pubmed ID
11254691DOI
10.4049/jimmunol.166.7.4370Scopus ID
2-s2.0-0035313052 (requires institutional sign-in at Scopus site) 69 CitationsAbstract
Minor histocompatibility Ags (minor H Ags) are substantial impediments to MHC-matched solid tissue and bone marrow transplantation. From an antigenic standpoint, transplantation between MHC-matched individuals has the potential to be remarkably complex. To determine the extent to which the immune response is simplified by the phenomenon of immunodominance, we used peptide/MHC tetramers based on recently discovered minor H Ags (H60, H13, and HY) and monitored in vivo CD8 T cell responses of female C57BL/6 mice primed with MHC-matched, but background-disparate, male BALB.B cells. CD8 T cells against H60 overwhelmed responses to the H13 and HY throughout primary and secondary challenge. H60 immunodominance was an inherent quality, overcoming a lower memory precursor frequency compared with that of H13 and evoking a T cell response with diverse TCRV beta usage. IFN-gamma staining examining congenically defined minor H Ags extended H60 dominance over additional minor H Ags, H28, H4, and H7. These four minor H Ags accounted for up to 85% of the CD8 T cell response, but H60 stood out as the major contributor. These findings show that immunodominance applies to antigenically complex transplantation settings in vivo and that the responses to the H60 minor H Ag dominates in this model. We suggest that immunodominant minor H Ags are those that result from the absence of a self analog.
Author List
Choi EY, Yoshimura Y, Christianson GJ, Sproule TJ, Malarkannan S, Shastri N, Joyce S, Roopenian DCAuthor
Subramaniam Malarkannan PhD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCD8-Positive T-Lymphocytes
Clone Cells
Cytotoxicity Tests, Immunologic
Epitopes, T-Lymphocyte
Female
Flow Cytometry
Immunization, Passive
Immunodominant Epitopes
Longitudinal Studies
Lymphocyte Activation
Lymphocyte Culture Test, Mixed
Male
Mice
Mice, Congenic
Mice, Inbred BALB C
Mice, Inbred C57BL
Minor Histocompatibility Antigens
Receptors, Antigen, T-Cell, alpha-beta
Skin Transplantation
Species Specificity
Spleen
