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Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations. Free Radic Biol Med 2012 Jan 01;52(1):1-6

Date

10/27/2011

Pubmed ID

22027063

Pubmed Central ID

PMC3911769

DOI

10.1016/j.freeradbiomed.2011.09.030

Scopus ID

2-s2.0-84655163961 (requires institutional sign-in at Scopus site)   1422 Citations

Abstract

The purpose of this position paper is to present a critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species. Where feasible, we have made recommendations for the use of alternate probes and appropriate analytical techniques that measure the specific products formed from the reactions between fluorescent probes and reactive oxygen and nitrogen species. We have proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results.

Author List

Kalyanaraman B, Darley-Usmar V, Davies KJ, Dennery PA, Forman HJ, Grisham MB, Mann GE, Moore K, Roberts LJ 2nd, Ischiropoulos H

Author

Balaraman Kalyanaraman PhD Professor in the Biophysics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Fluorescent Dyes
Hydrogen Peroxide
Oxidation-Reduction
Peroxynitrous Acid
Reactive Nitrogen Species
Reactive Oxygen Species
Spectrometry, Fluorescence
Staining and Labeling
Superoxides