Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations. Free Radic Biol Med 2012 Jan 01;52(1):1-6
Date
10/27/2011Pubmed ID
22027063Pubmed Central ID
PMC3911769DOI
10.1016/j.freeradbiomed.2011.09.030Scopus ID
2-s2.0-84655163961 (requires institutional sign-in at Scopus site) 1422 CitationsAbstract
The purpose of this position paper is to present a critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species. Where feasible, we have made recommendations for the use of alternate probes and appropriate analytical techniques that measure the specific products formed from the reactions between fluorescent probes and reactive oxygen and nitrogen species. We have proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results.
Author List
Kalyanaraman B, Darley-Usmar V, Davies KJ, Dennery PA, Forman HJ, Grisham MB, Mann GE, Moore K, Roberts LJ 2nd, Ischiropoulos HAuthor
Balaraman Kalyanaraman PhD Professor in the Biophysics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Fluorescent DyesHydrogen Peroxide
Oxidation-Reduction
Peroxynitrous Acid
Reactive Nitrogen Species
Reactive Oxygen Species
Spectrometry, Fluorescence
Staining and Labeling
Superoxides