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Delayed cardioprotection by isoflurane: role of K(ATP) channels. Am J Physiol Heart Circ Physiol 2002 Jul;283(1):H61-8

Date

06/14/2002

Pubmed ID

12063275

DOI

10.1152/ajpheart.01040.2001

Scopus ID

2-s2.0-0036306453   82 Citations

Abstract

Isoflurane mimics the cardioprotective effect of acute ischemic preconditioning with an acute memory phase. We determined whether isoflurane can induce delayed cardioprotection, the involvement of ATP-sensitive potassium (K(ATP)) channels, and cellular location of the channels. Neonatal New Zealand White rabbits at 7-10 days of age (n = 5-16/group) were exposed to 1% isoflurane-100% oxygen for 2 h. Hearts exposed 2 h to 100% oxygen served as untreated controls. Twenty-four hours later resistance to myocardial ischemia was determined using an isolated perfused heart model. Isoflurane significantly reduced infarct size/area at risk (means +/- SD) by 50% (10 +/- 5%) versus untreated controls (20 +/- 6%). Isoflurane increased recovery of preischemic left ventricular developed pressure by 28% (69 +/- 4%) versus untreated controls (54 +/- 6%). The mitochondrial K(ATP) channel blocker 5-hydroxydecanoate (5-HD) completely (55 +/- 3%) and the sarcolemmal K(ATP) channel blocker HMR 1098 partially (62 +/- 3%) attenuated the cardioprotective effects of isoflurane. The combination of 5-HD and HMR-1098 completely abolished the cardioprotective effect of isoflurane (56 +/- 5%). We conclude that both mitochondrial and sarcolemmal K(ATP) channels contribute to isoflurane-induced delayed cardioprotection.

Author List

Tonkovic-Capin M, Gross GJ, Bosnjak ZJ, Tweddell JS, Fitzpatrick CM, Baker JE

Authors

John E. Baker PhD Professor in the Surgery department at Medical College of Wisconsin
Zeljko J. Bosnjak PhD Professor in the Medicine department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Adenosine Triphosphate
Animals
Animals, Newborn
Benzamides
Cytoprotection
Decanoic Acids
Dose-Response Relationship, Drug
Heart
Hydroxy Acids
In Vitro Techniques
Isoflurane
Mitochondria
Myocardial Infarction
Myocardial Ischemia
Myocardium
Potassium Channel Blockers
Potassium Channels
Rabbits
Sarcolemma
Time Factors
Ventricular Function, Left
jenkins-FCD Prod-482 91ad8a360b6da540234915ea01ff80e38bfdb40a