Characterization of radical adducts formed during photochemical spin trapping in liposomes. Photochem Photobiol 1990 Aug;52(2):323-31
Date
08/01/1990Pubmed ID
2171003DOI
10.1111/j.1751-1097.1990.tb04188.xScopus ID
2-s2.0-0025469328 (requires institutional sign-in at Scopus site) 4 CitationsAbstract
There exists an urgent need to monitor radical reactions in biological membranes. With the exception of a few studies in model and natural membranes, most biological spin trapping reactions have been confined to homogeneous media. In the present work we have devised a methodology by which spin trapping reactions can be investigated in liposomes composed of a fully-saturated phospholipid, dimyristoylphosphatidylcholine. Using photochemical spin trapping, we have detected and characterized the formation and partitioning of spin adducts in liposomes. Benzophenone (a lipid-soluble photosensitizer) and benzoyl peroxide (a lipid-soluble radical initiator) have been used to generate free radicals from hydrogen donors during photolysis in liposomes. Both water-soluble and lipid-soluble spin traps have been used to trap the radicals.
Author List
Sheng PG, Feix J, Kalyanaraman BAuthors
Jimmy B. Feix PhD Professor in the Biophysics department at Medical College of WisconsinBalaraman Kalyanaraman PhD Professor in the Biophysics department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
BenzophenonesBenzoyl Peroxide
Dimyristoylphosphatidylcholine
Electron Spin Resonance Spectroscopy
Free Radicals
Liposomes
Models, Biological
Photolysis
Radiation-Sensitizing Agents