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Characterization of radical adducts formed during photochemical spin trapping in liposomes. Photochem Photobiol 1990 Aug;52(2):323-31

Date

08/01/1990

Pubmed ID

2171003

DOI

10.1111/j.1751-1097.1990.tb04188.x

Scopus ID

2-s2.0-0025469328 (requires institutional sign-in at Scopus site)   4 Citations

Abstract

There exists an urgent need to monitor radical reactions in biological membranes. With the exception of a few studies in model and natural membranes, most biological spin trapping reactions have been confined to homogeneous media. In the present work we have devised a methodology by which spin trapping reactions can be investigated in liposomes composed of a fully-saturated phospholipid, dimyristoylphosphatidylcholine. Using photochemical spin trapping, we have detected and characterized the formation and partitioning of spin adducts in liposomes. Benzophenone (a lipid-soluble photosensitizer) and benzoyl peroxide (a lipid-soluble radical initiator) have been used to generate free radicals from hydrogen donors during photolysis in liposomes. Both water-soluble and lipid-soluble spin traps have been used to trap the radicals.

Author List

Sheng PG, Feix J, Kalyanaraman B

Authors

Jimmy B. Feix PhD Professor in the Biophysics department at Medical College of Wisconsin
Balaraman Kalyanaraman PhD Professor in the Biophysics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Benzophenones
Benzoyl Peroxide
Dimyristoylphosphatidylcholine
Electron Spin Resonance Spectroscopy
Free Radicals
Liposomes
Models, Biological
Photolysis
Radiation-Sensitizing Agents