Spin traps: in vitro toxicity and stability of radical adducts. Free Radic Biol Med 2003 Jun 01;34(11):1473-81
Date
05/22/2003Pubmed ID
12757857DOI
10.1016/s0891-5849(03)00182-5Scopus ID
2-s2.0-0038030710 (requires institutional sign-in at Scopus site) 111 CitationsAbstract
We have evaluated the effects of DMPO, CMPO, EMPO, BMPO, and DEPMPO on functioning CHO cells and the stability of the radical adducts in the presence of cells. The potential toxic effects of the spin traps were measured by two estimates of cell viability (trypan blue exclusion and colony formation) and one of cell function (rate of oxygen consumption). We also studied the effects of the spin traps on colony formation in a second cell line, 9L tumor cells. Toxicity varied with the type of cell line and the parameter that was measured. In aqueous solutions the order of stability for all spin adducts was SO(3) > OH > CH(3), while in cell suspensions it was SO(3) > OH approximately CH(3). The radical adducts of the new spin traps have significantly increased stability as compared to DMPO. These results indicate that the new spin traps potentially offer increased stability of spin adducts in functioning cells. It also is clear that it is necessary to carry out appropriate studies of the stability and toxicity in the system that is to be studied for any particular use of these spin traps. It then should be feasible to select the spin trap(s) best suited for the proposed study.
Author List
Khan N, Wilmot CM, Rosen GM, Demidenko E, Sun J, Joseph J, O'Hara J, Kalyanaraman B, Swartz HMAuthor
Balaraman Kalyanaraman PhD Professor in the Biophysics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBrain Neoplasms
CHO Cells
Cell Division
Cell Survival
Colony-Forming Units Assay
Cricetinae
Cyclic N-Oxides
Drug Stability
Electron Spin Resonance Spectroscopy
Free Radicals
Half-Life
Kinetics
Oxygen Consumption
Rats
Spin Labels
Spin Trapping
Sulfites
Trypan Blue
Tumor Cells, Cultured
