Faculty Collaboration Database

Three-dimensional mapping of ozone-induced injury in the nasal airways of monkeys using magnetic resonance imaging and morphometric techniques. Toxicol Pathol 2007 Jan;35(1):27-40

Date

02/28/2007

Pubmed ID

17325970

DOI

10.1080/01926230601072343

Scopus ID

2-s2.0-33847312157 (requires institutional sign-in at Scopus site)   35 Citations

Abstract

Age-related changes in gross and microscopic structure of the nasal cavity may alter local tissue susceptibility as well as the dose of inhaled toxicant delivered to susceptible sites. This article describes a novel method for the use of magnetic resonance imaging, 3-dimensional airway modeling, and morphometric techniques to characterize the distribution and magnitude of ozone-induced nasal injury in infant monkeys. Using this method, we generated age-specific, 3-dimensional, epithelial maps of the nasal airways of infant Rhesus macaques. The principal nasal lesions observed in this primate model of ozone-induced nasal toxicology were neutrophilic rhinitis, along with necrosis and exfoliation of the epithelium lining the anterior maxilloturbinate. These lesions, induced by acute or cyclic (episodic) exposures, were examined by light microscopy, quantified by morphometric techniques, and mapped on 3-dimensional models of the nasal airways. Here, we describe the histopathologic, imaging, and computational biology methods developed to precisely characterize, localize, quantify, and map these nasal lesions. By combining these techniques, the location and severity of the nasal epithelial injury were correlated with epithelial type, nasal airway geometry, and local biochemical and molecular changes on an individual animal basis. These correlations are critical for accurate predictive modeling of exposure-dose-response relationships in the nasal airways, and subsequent extrapolation of nasal findings in animals to humans for determining risk.

Author List

Carey SA, Minard KR, Trease LL, Wagner JG, Garcia GJ, Ballinger CA, Kimbell JS, Plopper CG, Corley RA, Postlethwait EM, Harkema JR, Einstein DR

Author

Guilherme Garcia PhD Assistant Professor in the Biomedical Engineering department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Computational Biology
Imaging, Three-Dimensional
Macaca mulatta
Magnetic Resonance Imaging
Male
Nasal Cavity
Nasal Mucosa
Oxidants, Photochemical
Ozone
Rhinitis
Turbinates