Optical imaging of tissue mitochondrial redox state in intact rat lungs in two models of pulmonary oxidative stress. J Biomed Opt 2012 Apr;17(4):046010
Date
05/09/2012Pubmed ID
22559688Pubmed Central ID
PMC3380956DOI
10.1117/1.JBO.17.4.046010Scopus ID
2-s2.0-84865605652 (requires institutional sign-in at Scopus site) 34 CitationsAbstract
Ventilation with enhanced fractions of O(2) (hyperoxia) is a common and necessary treatment for hypoxemia in patients with lung failure, but prolonged exposure to hyperoxia causes lung injury. Ischemia-reperfusion (IR) injury of lung tissue is common in lung transplant or crush injury to the chest. These conditions are associated with apoptosis and decreased survival of lung tissue. The objective of this work is to use cryoimaging to evaluate the effect of exposure to hyperoxia and IR injury on lung tissue mitochondrial redox state in rats. The autofluorescent mitochondrial metabolic coenzymes nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) are electron carriers in ATP generation. These intrinsic fluorophores were imaged for rat lungs using low-temperature fluorescence imaging (cryoimaging). Perfused lungs from four groups of rats were studied: normoxia (control), control perfused with an mitochondrial complex IV inhibitor (potassium cyanide, KCN), rats exposed to hyperoxia (85% O(2)) for seven days, and from rats subjected to lung IR in vivo 24 hours prior to study. Each lung was sectioned sequentially in the transverse direction, and the images were used to reconstruct a three-dimensional (3-D) rendering. In KCN perfused lungs the respiratory chain was more reduced, whereas hyperoxic and IR lung tissue have a more oxidized respiratory chain than control lung tissue, consistent with previously measured mitochondrial dysfunction in both hyperoxic and IR lungs.
Author List
Sepehr R, Staniszewski K, Maleki S, Jacobs ER, Audi S, Ranji MAuthors
Said Audi PhD Professor in the Biomedical Engineering department at Marquette UniversityMahsa Ranji PhD Assistant Professor in the Electrical Engineering department at University of Wisconsin - Milwaukee
MESH terms used to index this publication - Major topics in bold
Analysis of VarianceAnimals
Calibration
Cold Temperature
Flavin-Adenine Dinucleotide
Histological Techniques
Hyperoxia
Image Processing, Computer-Assisted
Lung
Lung Injury
Male
Microscopy, Fluorescence
Mitochondria
Models, Biological
NAD
Oxidation-Reduction
Oxidative Stress
Rats
Rats, Sprague-Dawley
Reperfusion Injury
