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Failure of exogenous serotonin to inhibit the release of 3H-serotonin induced by electrical field stimulation in superfused goldfish brain slices. Gen Pharmacol 1982;13(2):157-60

Date

01/01/1982

Pubmed ID

7095394

DOI

10.1016/0306-3623(82)90072-6

Scopus ID

2-s2.0-4243303071 (requires institutional sign-in at Scopus site)

Abstract

1. Goldfish brain slices preloaded with 3H-serotonin were superfused with a solution of Krebs-Ringer phosphate at 30 degrees C in the presence of 1 microM fluoxetine, a serotonin uptake blocker. 2. The effects of exogenous serotonin, Ca2+ absence and Ca2+ chelation on basal and electrically-evoked tritium overflow were investigated. 3. Exogenous 1 or 10 microM serotonin did not alter the stimulated tritium overflow, but this was reduced to 44% and to 31% of the control value when the superfusion was performed in Ca2+-free and in Ca2+-free-EGTA conditions. 4. Neither Ca2+ absence, Ca2+ chelation or 1 microM exogenous serotonin altered basal tritium efflux; however, the latter was significantly increased when the superfusion was performed in the presence of 10 microM serotonin.

Author List

Figueroa HR, Newton DK, Yurgens PB, Olcese JM, Hall TR

Author

Debra K. Newman PhD Investigator in the Blood Research Institute department at BloodCenter of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Brain
Calcium
Electric Stimulation
Feedback
Goldfish
In Vitro Techniques
Receptors, Serotonin
Serotonin
Synapses